| 利用飞行时间质谱进行蛋白质和多肽C端序列测定 |
| |
| 引用本文： | 陈平,梁宋平. 利用飞行时间质谱进行蛋白质和多肽C端序列测定[J]. 湖南师范大学自然科学学报, 2001, 24(1): 58-61 |
| |
| 作者姓名： | 陈平 梁宋平 |
| |
| 作者单位： | 湖南师范大学生物化学与分子生物学系, |
| |
| 基金项目： | 国家自然科学基金资助项目!(39990 6 0 0 ) |
| |
| 摘 要： | 用羧肽酶Y水解蛋白质和多肽,结合飞行时间质谱（MALDI－TOF－MS）测定酶解后缩短肽的质量,根据相邻两肽峰的质量差别读出蛋白质和多肽的C端序列,在pmol水平下测定了人促肾上腺皮质激素片段以及人血管紧张肽片段,其中人促上腺皮质激素片段测到了C端15个氨基酸残基的顺序。
|
| 关 键 词： | 飞行时间质谱 C端测序 羧肽酶Y 蛋白质 多肽 序列测定 结构 生理活性 |
| 文章编号： | 1000-2537(2001)01-0058-04 |
| 修稿时间： | 2000-11-10 |
C-terminal Sequencing of Protein and Peptide by MALDI-TOF Mass Spectrometry |
| |
| CHEN Ping,LIANG Song ping. C-terminal Sequencing of Protein and Peptide by MALDI-TOF Mass Spectrometry[J]. Journal of Natural Science of Hunan Normal University, 2001, 24(1): 58-61 |
| |
| Authors: | CHEN Ping LIANG Song ping |
| |
| Abstract: | Using of matrix assisted laser desorption/ionization time of flight (MALDI TOF) mass spectrometry coupled with carboxypeptidase Y digestion for the analysis of C terminal peptide ladder by direct determination of the mixture of truncated peptide, the sequence of C terminus can be read out simplely from the mass differences between adjacent peptide peaks on the mass spectrum. The C terminal sequence of ACTH fragment 1 39 and Angiotensin I were determined. The C terminal sequence of ACTH fragment 1 39 yielded sequence information 15 amino acids from the C terminus without gaps. |
| |
| Keywords: | MALDI TOF MS C terminal sequencing Carboxypeptidase Y |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
