微生物生长吸附规律及防蜡机理实验研究

用浊度法测定了江苏油田现场所用3株防蜡微生物的生长曲线,用染色、拍照、计数法研究了实验微生物在生长过程中的吸附规律、吸附平衡时间和稀释时的吸附变化规律,用测润湿角方法研究了微生物作用时表面润湿性的变化。结果表明,实验微生物生长时具有明显的延滞期、对数生长期和稳定期,温度升高使延滞时间增长,生长速度加快,而且壁面上的吸附密度与菌浓度不成正比关系,吸附平衡时间为1～1.5h,将菌浓度逐次稀释到10个/mL时,壁面吸附密度仍很高;微生物作用可加速壁面润湿性变化,使油相粘附功大幅减小,从而不利于蜡在管壁上沉积;微生物作用可使蜡组分减少和含量降低。

Experimental research of microbial adsorption law and paraffin control mechanism

The growth curves of three paraffin control strains were determined by the turbid method. The laws of microbial adsorption on surface during growth, the balance time and the density change as concentrations diluted were studied by coloring, photographing and counting. Using measuring wetting angle of steel in microbial liquids, wettability changes were researched. The results show that the growth curves of three strains have typical characteristics of prolong, logarithm and steady period, and are also affected by temperature. The density of microbial adsorption on surface does not vary directly as the concentration, the balance time of adsorption is 1 to 1.5 hours and the density is high during diluted. The wettability of steel changes rapidly with microbe acting and the cohesion work of oil decreases greatly, which contributes to paraffin control in oil well. The microbe can decrease the content of the paraffin and change the characteristics of paraffin crystal.