大花君子兰的胚培养及其胚状体的诱导

大花君子兰幼胚培养,其胚状体采用MS培养基(其中大量元素减半),附加ZT1毫克/升,NAA0.2毫克/升,IAA0.2毫克/升,蔗糖1.5％、琼脂0.65％、pH5.8％,在温度25±1℃、光照1200 Lx(10小时/天)下培养20天后即诱导出芽,然后转入壮苗发根培养基:MS培养基(其中大量元素减半)、附加BA0.05毫克/升、IBA0.2毫克/升、IAA0.6毫克/升,蔗糖、琼脂、pH及培养条件同上。培养三个星期后即长出叶片并发根2—3条。将试管苗鳞茎状部分切成0.5—1 cm见方的小块,接入壮苗发根培养基中,经2—3次(每次20天)继代培养形成8—9个胚状体,然后移至无激素的MS培养基中培养(培养条件同上),19天后转入壮苗发根培养基中,16天后相继长出根和叶,成为壮苗。最后经炼苗移栽至花盆,成活率可达100％。

Tissue Culture of Cliuia Embryo and Induction of Embryoid

In the present paper it describes the tissue culture of Clivia and induction of embryoid.The early embryoes, deriving from the eighty-day-old fruits on a five-year-old maternal plant, are cultivated on the modified MS media, in which the amount of macroelements has been reduced by half, and supplemented with ZT 1mg/l, NAA 0.2mg/l, IAA0.2mg/l, sucrose 1.5%, agar 0.65%, pH5.8. After induced to germinate for 20 days, the duds are transfered into the basic media mentioned above and supplemented with BA 0.05mg/l, IBA0.2mg/l, IAA0.6mg/l, sucrose 1.5%, agar 0.6%, pH5.8. Affter three weeks the regenerated plants with shining fatty leaves and strong white roots are obtained on this sprout-strengthen and rootinducing media.The slices of bulb of regenerated plants are cultivated on the sprout-strengthening and root-inducing media. After cultivated for two or three generations, 8 or 9 embryoids are directly produced from the slices of bulb. Then they are transfered and cultivated on MS media without any phytohormone. For 19 days the embryoids change from small to big, pale-yellow to green. Cultivated again on the sprout-strengthening and root-inducing media for 16 days, the embryoids graduaily grow into the matured regenerated-plants.The regenerated plants obtained by these method are transplanted on the cultivating bed. The time when the plastic films are uncovered are gradually prolonged 3 days later. The plastic films can be uncovered completely 7 days later. After 10 days they can be transplanted in the flower-pot filled with sandy soil and humus soil. The survival rate can reach 100%.