| BIV－LTR中存在负调控区 |
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| 引用本文： | 徐宏涛,梁臣,刘淑红,陈启民,耿运琪.BIV－LTR中存在负调控区[J].南开大学学报,1996(4). |
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| 作者姓名： | 徐宏涛 梁臣 刘淑红 陈启民 耿运琪 |
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| 作者单位： | 南开大学生命科学学院 |
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| 摘 要： | 为研究ＢＩＶ－ＬＴＲ调控序列的功能，将ＬＴＲ部分ＤＮＡ序列缺失，与荧光素酶基因相连构建质粒ｐＤ－３１９－Ｌｕｃ，ｐＤ－２６１－Ｌｕｃ，ｐＤ－１８１－Ｌｕｃ，ｐＤ－２－Ｌｕｃ，ｐＤ＋３２－Ｌｕｃ，ｐＤ－３１９－２６１－Ｌｕｃ，ｐＤ－１８１＋３２－Ｌｕｃ．通过磷酸钙共沉淀法将上述质粒及ｐＢＬＴＲ－Ｌｕｃ分别转染小牛肺细胞（ＦＢＬ），检测转染细胞裂解物荧光素酶活性，比较各质粒所克隆的ＢＩＶ－ＬＴＲ表达水平，发现－２６１位上游区存在负调控区，Ｒ区存在抑制ＬＴＲ表达的序列．
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| 关 键 词： | 牛免疫缺陷病毒 长末端重复区 荧光素酶 反式激活因子 |
NEGATIVE REGULATORY ELEMENTS IN BIV LTR |
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| Xu Hongtao,Liang Chen,Liu Shuhong,Chen Qimin,Geng Yunqi.NEGATIVE REGULATORY ELEMENTS IN BIV LTR[J].Acta Scientiarum Naturalium University Nankaiensis,1996(4). |
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| Authors: | Xu Hongtao Liang Chen Liu Shuhong Chen Qimin Geng Yunqi |
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| Abstract: | To Study the functions of BIV LTR regulatory; elements, the LTR was partially deleted and linked to luciferase gene, hence,we constructed a series of plasmids: pD-319-Luc,pD-261-Luc,pD-181-Luc,pD-2-Luc,PD+32-Luc, pD-319-261-Luc and pD-181+32-Luc. Using the phosphate-calcium co-transfection technique, these plasmids and pBLTR-Luc was transfected into Fetal bovine lung cell (FBL),respectively.Through the detection of luciferase activities in the lysised debries of the transfected cells,we compared the expression levels of each cloned D BIV LTR,and found that DNA sequence upstream of-261 site can repress the expression of LTR,meanwhile,there is a negative regulatory region in the R region of LTR as well. |
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| Keywords: | Bovine Immunodeficiency Virus long terminal repeats luciferase transactivator |
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