| 叶用莴苣组织培养研究 |
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| 引用本文： | 高昌勇,尚宏芹.叶用莴苣组织培养研究[J].菏泽学院学报,2004,26(4):61-62. |
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| 作者姓名： | 高昌勇 尚宏芹 |
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| 作者单位： | 菏泽学院生物系,山东,菏泽,274015 |
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| 摘 要： | 以叶用莴苣(LactucasativaL)栽培品种的幼嫩叶片为实验材料,对叶用莴苣的组织培养进行了研究,探讨愈伤组织的形成、愈伤组织分化成芽和生根的条件.结果如下6BA和NAA的浓度影响叶用莴苣愈伤组织的形成、分化和生根培养,诱导愈伤组织的最适培养基为MS+6BA(0.5mg/L)+NAA(0.1mg/L),最适分化培养基为MS+6BA(0.5mg/L)+NAA(1.0mg/L),最适生根培养基为1/2MS+NAA(0.1mg/L).
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| 关 键 词： | 叶用莴苣 愈伤组织 芽分化 |
| 文章编号： | 1003-6318(2004)04-0061-02 |
| 修稿时间： | 2004年6月11日 |
A Study on Tissue Culture of Lettuce |
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| GAO Chang-yong,SHANG Hong-qin.A Study on Tissue Culture of Lettuce[J].Journal of Heze University,2004,26(4):61-62. |
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| Authors: | GAO Chang-yong SHANG Hong-qin |
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| Abstract: | The infancy leaf of lettuce was taken as explants and cultured on MS medium composed of different concentration of 6-BA and NAA. The induction and differentiation of callus, buds and shoots were disscused. The result showed that the concentration of 6-BA and NAA effected the formation and differentiation of callus of lettuce leaf,the most suitable culture medium to the formation of callus was MS+6-BA(0.5 mg/L)+NAA(0.1 mg/L), the most suitable culture medium to the differentiation of bud was 6-BA(0.5 mg/L)+NAA(1.0 mg/L), and the most suitable culture medium to growth of shoots was 1/2MS+NAA(0.1 mg/L). |
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| Keywords: | Lettuce callus buds differentiation |
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