| 人参HPLC指纹图谱的研究 |
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| 引用本文： | 阎正,苑若瑶,王春云,蔡立鹏,王继坤. 人参HPLC指纹图谱的研究[J]. 河北大学学报(自然科学版), 2009, 29(3): 278. DOI: 10.3969/j.issn.1000-1565.2009.03.014 |
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| 作者姓名： | 阎正 苑若瑶 王春云 蔡立鹏 王继坤 |
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| 作者单位： | 1.河北大学,化学与环境科学学院,河北,保定071002； 2.北京中防昊通防伪技术中心,北京,100025 |
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| 摘 要： | 建立了人参的高效液相指纹图谱.研究了不同的预处理方法、流动相组成、最佳检测波长、色谱柱温度等操作因素对人参指纹谱图的影响,并对不同产地和种类的人参药材的指纹图谱进行了比较.色谱条件为:色谱柱为不锈钢填充柱(Inertsil ODS-3,250 mm×4.6 mm,5 μm);检测波长为203 nm;流动相为乙腈-(质量分数为0.05%)磷酸水溶液梯度洗脱;流速1 mL/min ;分析时间90 min;柱温为30 ℃.
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| 关 键 词： | 人参 HPLC 指纹图谱 |
Study of Fingerprint of Ginseng by HPLC |
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| YAN Zheng,YUAN Ruo-yao,WANG Chun-yun,CAI Li-peng,WANG Ji-kun. Study of Fingerprint of Ginseng by HPLC[J]. Journal of Hebei University (Natural Science Edition), 2009, 29(3): 278. DOI: 10.3969/j.issn.1000-1565.2009.03.014 |
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| Authors: | YAN Zheng YUAN Ruo-yao WANG Chun-yun CAI Li-peng WANG Ji-kun |
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| Abstract: | This paper described an analytical method of fingerprint of Ginseng by HPLC.We studied different sample pretreatment,detection wavelength,mobile phase,temperature and compared the fingerprint chromatograms of Ginseng from six different areas and kinds.Inertsil ODS-3 column(250 mm×4.6 mm,5 μm) was used with the mixture of acetonitrile-0.05% phosphoric acid as mobile phase;the detection wavelength was 203 nm;the flow rate was 1 mL/min and the column temperature was 30 ℃. |
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| Keywords: | HPLC |
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