医用藻酸盐敷料体外降解的研究

研究藻酸盐医用敷料体外降解的规律及不同降解时间其产物对内皮细胞增殖情况的影响,为藻酸盐医用敷料在临床使用中提供参考。以PBS缓冲液(模拟体液环境)为降解介质,对藻酸盐敷料进行体外降解;用藻酸盐敷料的失重率、分子质量变化表征本体的变化规律;用降解液中降解产物的糖醛酸含量变化表征降解产物的变化规律。将降解液与内皮细胞共培养,用MTT法检测内皮细胞增殖情况。藻酸盐敷料在模拟体液环境条件下,可以被降解,42 d失重率为61.2%,降解过程中其分子质量逐渐减小,最终产物为糖醛酸,其浓度不断增大。不同时间的降解产物均能促进内皮细胞的增殖。藻酸盐医用敷料体外降解比较快,完全降解的最终产物为糖醛酸,其降解液可以促进内皮细胞的增殖、分化,提高细胞新陈代谢能力,增加新细胞的再生速度,促进伤口的愈合。

The Study of the Degradation for Medical Alginate Dressings in Vitro

Objective To study the degradation of medical alginate dressings in PBS buffer and the effect of degradation products on proliferation of endothelial cells as well as to provide the reference for medical alginate dressings in clinical use. Methods The degradation was prepared by immersing the medical alginate dressings in PBS buffer (SBF environment) under aseptic condition.The weight loss rate and molecular weigh were measured.On the other hand,the uronate concentration of degradation products were measured. The human umbilical vein endothelial cells ECV-304 was used and cultured with the degradation fluid. Cell proliferation was detected with MTT assay. Results Medical alginate dressings in a simulated body fluid environmental conditions can be degraded.The weight loss was 61.2FfFf in 42d.The molecular weight decreased with the increasing of degradation time.The final product of medical alginate dressings was uronic acid and the concentration of uronic acid increased with the increasing of degradation time.As well as the degradation products at different time could promote the proliferation of endothelial cells. Conclusion Medical alginate dressings can be degradated faster in vitro degradation and the final degradation product is uronic acid.The degradation product of medical alginate dressings can promote the endothelial cell proliferation, differentiation.Which can improve metabolism of body,increasing the speed of new cell regeneration and promoting wound healing.