| 反相高效液相法测定八角茴香果实中的莽草酸 |
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| 引用本文： | 李伟,曹庸,魏华,周冬武.反相高效液相法测定八角茴香果实中的莽草酸[J].吉首大学学报(自然科学版),2008,29(1):104-106. |
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| 作者姓名： | 李伟 曹庸 魏华 周冬武 |
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| 作者单位： | (1.吉首大学生物资源与环境科学学院，湖南 吉首 416000；2.湖南省林产化工重点实验室，湖南 张家界 427000；3.华南农业大学食品科学学院，广东 广州 510642) |
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| 摘 要： | 用反相高效液相法在Waters Symmetry C18柱(5 μm,4.6 mm×150 mm i.d)上测定八角茴香果实中的莽草酸.工作参数为：流速0.5 mL/min；进样体积2 μL；检测波长220 nm；柱温30 ℃±1 ℃；流动相0.01 mol/L K2HPO4磷酸盐缓冲液(pH值为3，用磷酸调配)和体积分数3%甲醇.平均回收率为97.76 %，RSD为1.937 %(n=5).
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| 关 键 词： | 反相高效液相色谱法 莽草酸 八角茴香 |
| 文章编号： | 1007-2985(2008)01-0104-03 |
| 修稿时间： | 2007年10月28 |
Reversed-Phase HPLC Determination of Shikimic Acid in Illicium Verum Hook.F. |
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| LI Wei,CAO Yong,WEI Hua,ZHOU Dong-wu.Reversed-Phase HPLC Determination of Shikimic Acid in Illicium Verum Hook.F.[J].Journal of Jishou University(Natural Science Edition),2008,29(1):104-106. |
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| Authors: | LI Wei CAO Yong WEI Hua ZHOU Dong-wu |
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| Institution: | (1.College of Biology and Environmental Science,Jishou University,Jishou,Hunan 416000,China;2.Key Laboratory of Hunan Forest Products and Chemical Industry Engineering,Zhangjiajie Campus of Jishou University,Zhangjiajie Hunan 427000,China;3.College of Food Science,South China Agriculture University,Guang zhou 510642,China) |
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| Abstract: | OBJECTIVE To establish a RP－HPLC method for the determination of Shikimic Acid in Illicium verum Hook.f.METHODS In RP－HPLC analysis,a Waters Symmetry C18 column (5 μm,4.6 mm×150 mm,i.d.) was selected as separation column at 30 ℃±1 ℃.The mobile phase consisted of 0.01 mol/L K2HPO4 phosphate buffer (pH=3,I =0.17 mol/L) and 3% methanol.The flow rate was 0.5 mL/min and the wavelength of measurement was 220 nm.The average recovery was 97.76% with RSD of 1.937% (n=5).CONCLUSION The method was proved to be quick,simple,and highly sensitive and can be useful in Shikimic Acid medicines quality control. |
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| Keywords: | RP－HPLC Illicium verum Hook f Shikimic Acid |
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