Mitochondrial and cytosolic glutathione after depletion by phorone in isolated hepatocytes |
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Authors: | F J Romero S Soboll H Sies |
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Institution: | (1) Institut für Physiologische Chemie 1, Universität Düsseldorf, Moorenstrasse 5, D-4000 Düsseldorf 1, (Federal Republic of Germany);(2) Present address: Depto. Bioquímica, Facultad de Medicina, Universidad de Valencia, Av. Blasco Ibañez 17, 10 Valencia, Spain |
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Abstract: | Summary The glutathione content of cytosol and mitochondria of isolated hepatocytes was depleted by addition of a low concentration of phorone (0.5 mM) by 75% and 40% respectively. Different rates of replenishment indicate metabolic separation of cytosolic and mitochondrial glutathione pools. The release from hepatocytes occurred at a rate of about 8 nmol/g wet weight/min, both in controls and after phorone depletion.This work was supported by the Ministerium für Wissenschaft und Forschung. Nordrhein-Westfalen, and by Deutsche Forschungsgemeinschaft. |
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