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Assembly of microarrays for genome-wide measurement of DNA copy number. |
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| Authors: | A M Snijders N Nowak R Segraves S Blackwood N Brown J Conroy G Hamilton A K Hindle B Huey K Kimura S Law K Myambo J Palmer B Ylstra J P Yue J W Gray A N Jain D Pinkel D G Albertson |
| Institution: | Comprehensive Cancer Center, University of California San Francisco, San Francisco, California 94143, USA. |
| Abstract: | We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05-0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds. |
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