| 乙肝病毒表面抗原基因植物表达载体的构建 |
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| 引用本文： | 赵春晖 王荣. 乙肝病毒表面抗原基因植物表达载体的构建[J]. 辽宁师范大学学报(自然科学版), 1999, 22(3): 240-244 |
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| 作者姓名： | 赵春晖 王荣 |
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| 作者单位： | [1]辽宁师范大学 [2]中国科学院 |
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| 摘 要： | 报道了将乙型肝炎病毒（ａｄｗ亚型）表面抗原（ＨＢｓＡｇ）基因和ＨＢＡｇ及其前导序列（ＨＢｓＡｇ＋ｐｒｅＳ２）基因分别插入植物表达载体ｐＲｏＫⅡ的ＣａＭＶ３５Ｓ启动子下游，构建为重组质粒ｐＲＨＢ和ｐＲＰ，并将其分别导入农杆菌ＬＢＡ４４０４中。
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| 关 键 词： | 前导序列 植物表达载体 乙病毒 HBsAg基因 |
Construction of Plant Expression Vectors of Human Hepatitis Virus Surface Antigen Gene |
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| ZHAO Chun hui ,FENG Bin ,WANG Guan lin ,WANG Rong ,TIAN Po. Construction of Plant Expression Vectors of Human Hepatitis Virus Surface Antigen Gene[J]. Journal of Liaoning Normal University(Natural Science Edition), 1999, 22(3): 240-244 |
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| Authors: | ZHAO Chun hui FENG Bin WANG Guan lin WANG Rong TIAN Po |
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| Affiliation: | ZHAO Chun hui 1,FENG Bin 1,WANG Guan lin 1,WANG Rong 2,TIAN Po 2 |
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| Abstract: | Hepatitis B Virus (HBV) cause not only human acute and chronic hepatitis,but also the original liver cancer.There is no effective treatment for chronic hepatitis B,so control and prevention become indispensable.We construct plant expression vectors (pRoKII) with hepatitis B surface antigen gene(HBsAg) alone or with it conbined with preS,and introduce the recombinant plasmids into Agrobacterium tumenfaciens. Now,tomato plants genetically transformed by Agrobacterium tumenfaciens were obtained. |
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| Keywords: | hepatitis B Virus surface antigen preS plant expression vectors |
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