| 紫外线诱变康宁木霉提高酶活力的研究 |
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| 引用本文： | 李爱华,李耀忠,宋莉莉. 紫外线诱变康宁木霉提高酶活力的研究[J]. 宁夏大学学报(自然科学版), 2003, 24(4): 389-391 |
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| 作者姓名： | 李爱华 李耀忠 宋莉莉 |
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| 作者单位： | 1. 宁夏大学,生命科学学院,宁夏,银川,750021
2. 宁夏大学后勤管理处,宁夏,银川,750021 |
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| 基金项目： | 国家科技攻关计划项目(2002BA901A31),宁夏"十五"重点科技攻关项目(2001 004 02) |
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| 摘 要： | 通过对康宁木霉菌种的诱变、筛选和固体发酵培养,研究了培养基、培养时间、诱变时间及pH值对诱变菌所产酶的活力的影响．结果表明,康宁木霉经诱变5min,在pH为6．0的玉米秸PDA培养基上培养72h,产生的C1酶和Cx酶的酶活力较高,C1酶的酶活力由73u／g提高到176u／g,增加了2．41倍,Cx酶的酶活力由798u／g提高到2069u／g,增加了2．59倍．
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| 关 键 词： | 紫外线 线诱变 康宁木霉 酶活力 康氏木霉 纤维素酶 |
| 文章编号： | 0253-2328(2003)04-0389-03 |
| 修稿时间： | 2003-08-18 |
The study of improving the activity of Trichoderma koningii by ultraviolet mutagenesis |
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| LI Ai-hua,LI Yao-zhong,SONG Li-li. The study of improving the activity of Trichoderma koningii by ultraviolet mutagenesis[J]. Journal of Ningxia University(Natural Science Edition), 2003, 24(4): 389-391 |
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| Authors: | LI Ai-hua LI Yao-zhong SONG Li-li |
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| Affiliation: | LI Ai-hua~1,LI Yao-zhong~2,SONG Li-li~1 |
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| Abstract: | Culture medium, culture time, induction time and medium pH were studied in this experiment about the effects on the activity of Trichoderma koningii-enzyme. The results showed that Trichoderma koningii was inducted for 5 min, then cultured for 72 h in the fodder medium that pH was 6.0, the activity of C_1 and C_x was higher than before, and the activity of C_1 was improved from 73 u/g to 176 u/g, which were improved by 2.41 times, the activity of C_x was improved from 798 u/g to 2 069 u/g, which were improved respectively 2.59 times. |
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| Keywords: | activity of enzyme Trichoderma koningii ultraviolet ray mutagenesis |
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