| 从大肠杆菌中获得高浓度质粒DNA的方法 |
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| 引用本文： | 李海霞.从大肠杆菌中获得高浓度质粒DNA的方法[J].实验室科学,2012,15(1):108-110. |
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| 作者姓名： | 李海霞 |
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| 作者单位： | 河南农业大学生命科学学院,河南郑州,450002 |
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| 摘 要： | 通过改进菌体的培养以及质粒小提方法,可以简便快捷地得到浓度很高的质粒DNA,经琼脂糖凝胶电泳、紫外分光光度法、酶切、PCR的验证,所提质粒可以应用于分子生物学的研究,特别是可以满足瞬时基因枪转化的要求。
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| 关 键 词： | 质粒 浓度 菌体培养 |
Method of preparing high-concentration plasmid DNA from E. coli |
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| LI Hai-xia.Method of preparing high-concentration plasmid DNA from E. coli[J].Laboratory Science,2012,15(1):108-110. |
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| Authors: | LI Hai-xia |
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| Institution: | LI Hai-xia ( College of Life Science, Henan Agricuhural University, Zhengzhou 450002, China) |
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| Abstract: | High-quality plasmid DNA is easily and quickly prepared by changing condition of bacteria cultivation and deyword step of extraction. The plasmid is identified by gel electrophoresis, ultraviolet spectrophotometry , restrictive enzyme digestion and PCR. The DNA can meet the demand of molecular biological research, especialy satisfied demand of particle bombardment transformation . |
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| Keywords: | plasmid concentration bacteria cultivation |
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