| “转氨基作用”实验方法的改进 |
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| 引用本文： | 张宝珠,赵玉红,李欣,张金红,蔺宝珠.“转氨基作用”实验方法的改进[J].实验室科学,2012,15(1):111-114. |
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| 作者姓名： | 张宝珠 赵玉红 李欣 张金红 蔺宝珠 |
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| 作者单位： | 南开大学生命科学学院,天津,300071 |
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| 摘 要： | 用改进的无水乙醇∶水∶尿素∶氨水配方做为展层剂代替传统的展层剂———饱和酚来检测氨基移换作用后的氨基酸含量。改进后的实验方法降低了苯酚对人体的危害,简化了实验步骤,降低了实验成本,取得了良好的实验结果,绿色环保。
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| 关 键 词： | 展层剂 绿色环保 实验教学 |
Improvement of the methods on transamination for basic biochemical experiment |
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| ZHANG Bao-zhu
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ZHAO Yu-hong
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LI-Xin
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ZHANG Jin-hong
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LIN Bao-zhu.Improvement of the methods on transamination for basic biochemical experiment[J].Laboratory Science,2012,15(1):111-114. |
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| Authors: | ZHANG Bao-zhu ZHAO Yu-hong LI-Xin ZHANG Jin-hong LIN Bao-zhu |
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| Institution: | ( Colloge of Life Science, Nankai University, Tianjin 300071, China ) |
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| Abstract: | Useing improved absolute alcohol： water： urea： ammonia water as developing reagent replaces traditional saturant phenol to detect the amino acids content of the transamination. The improved methods reduce the hazard of phenol. It simplifies experimental procedure, cuts duwn the cost of the experiment and gets very good results of the experiment. It is green environmental protection. |
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| Keywords: | developing reagent environmental protection experimental teaching |
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