Influence of He-Ne laser irradiation on the excision repair of cyclobutyl pyrimidine dimers in the wheat DNA |
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Authors: | Rong Han Xunling Wang Ming Yue |
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Institution: | (1) School of Life Science, Northwest University, 710069 Xi’an, China;(2) School of Life Science, Shanxi Normal University, 041004 Linfen, China;(3) School of Life Science, Lanzhou University, 730000 Lanzhou, China |
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Abstract: | Using agarose gel electrophoresis and T4-endodeoxyribonuclease-V, which is a kind of restriction endonuclease of cyclobutyl
pyrimidine dimer (CPD), the impacts of He-Ne laser (5 mW · mm−2) irradiation on DNA excision repair capacity in damaged wheat cells induced by enhanced ultraviolet-B (10.08 kJ ·m−2 ·d−1) radiation were studied. The results indicated that the content of endonuclease sensitive sites (ESS) was reduced by He-Ne
laser irradiation, which formed in cells irradiated by enhanced ultraviolet-B. With the irradiation of He-Ne laser, the excision
of CPDs and the reduction of single strand breaks (SSB) contents which were the endonuclease sensitive sites (ESS) digested
by T4-endodeoxyribonuclease-V had been stimulated in the wheat cells. |
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Keywords: | wheat ultraviolet-B He-Ne laser DNA excision repair |
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