| 苯酚-硫酸法测定藏木香中多糖含量 |
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| 引用本文： | 哈文秀,利毛才让,热增才旦. 苯酚-硫酸法测定藏木香中多糖含量[J]. 青海师范大学学报(自然科学版), 2011, 27(1): 35-37 |
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| 作者姓名： | 哈文秀 利毛才让 热增才旦 |
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| 作者单位： | 青海师范大学民师院化学系,青海西宁810008 [2]青海大学医学院中藏药研究中心,青海砥宁810001 |
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| 摘 要： | 采用传统的水煮醇沉法从藏木香中提取可溶性多糖,通过Sevag法脱蛋白后,以葡萄糖为对照品,使用苯酚-硫酸法测定多糖含量.结果表明,在波长486nm处测定吸光度,10-100μg/mL范围内吸光度与被测含量之间具有良好的线性关系,藏木香中多糖的含量为64.37%.
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| 关 键 词： | 藏木香 多糖 苯酚-硫酸法 |
Determination of the Content of Polysaccharide in Inula Helenium by Phenol-Sulfuric Acid Method |
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| HA Wen-xiu,LI Mao-cai-rang,RE Zeng-cai-dan. Determination of the Content of Polysaccharide in Inula Helenium by Phenol-Sulfuric Acid Method[J]. Journal of Qinghai Normal University(Natural Science Edition), 2011, 27(1): 35-37 |
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| Authors: | HA Wen-xiu LI Mao-cai-rang RE Zeng-cai-dan |
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| Affiliation: | HA Wen-xiu1,LI Mao-cai-rang1,RE Zeng-cai-dan2(1.Department of Nationality Chemistry,Qinghai Normal University,Xining 810008,China,2.Research Center of Chinese and Tibetan Medicine,Qinghai University Medical College,Xining 810001,China) |
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| Abstract: | The soluble polysaccharides in Inula helenium were extracted by the traditional method of boiling with water and depositing in alcohols.After removing proteins,the content of polysaccharides was determined by phenol-sulfuric acid method with glucose as st |
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| Keywords: | inula helenium polysaccharides phenol-sulfuric acid method |
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