| 天花粉蛋白基因的克隆、表达和活性鉴定 |
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| 引用本文： | 蔡煊,胡拥军,徐洪,俞吉安,孙兵.天花粉蛋白基因的克隆、表达和活性鉴定[J].上海交通大学学报,2002,36(10):1503-1505. |
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| 作者姓名： | 蔡煊 胡拥军 徐洪 俞吉安 孙兵 |
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| 作者单位： | 1. 上海交通大学,生命科学技术学院,上海,200030
2. 中国科学院,上海生物化学和细胞生物学研究所,上海,200030 |
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| 摘 要： | 用PCR（Polymerase Chain Reaction)扩增出天芬粉蛋白（Trichosanthin,TCS)基因全长序列，插入表达载体，经双酶切鉴定及测序分析，表明阅读框正确，表达His-TCS活性融合蛋白并纯化天花粉蛋白，鉴定出其具有RNA N－糖苷酶活性和抗原抗体结合活性。
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| 关 键 词： | 天花粉蛋白 基因克隆 分离纯化 生物活性 基因表达 RNA N－糖苷酶活性 抗原抗体结合活性 |
| 文章编号： | 1006-2467(2002)10-1503-03 |
| 修稿时间： | 2001年9月1日 |
Cloning, Expression and Characterization of Trichosanthin Gene |
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| CAI Xuan ,HU Yong jun ,XU Hong ,YU Ji an ,SUN Bing.Cloning, Expression and Characterization of Trichosanthin Gene[J].Journal of Shanghai Jiaotong University,2002,36(10):1503-1505. |
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| Authors: | CAI Xuan HU Yong jun XU Hong YU Ji an SUN Bing |
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| Institution: | CAI Xuan 1,HU Yong jun 2,XU Hong 2,YU Ji an 1,SUN Bing 2 |
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| Abstract: | TCS (Trichosanthin) gene was cloned into an expression vector. The plasmid was extracted and digested with restriction enzymes. The result of the sequence analysis shows the TCS whole length gene is obtained. The TCS protein was expressed and purified. The TCS protein's biological activity of RNA N glycosidase was characterized. ELISA shows that this protein can bind with specific antibody TE1(IgE). |
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| Keywords: | trichosanthin gene clone protein expression and purification biological activity |
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