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A method for injection and transplantation of nuclei and cells inDrosophila eggs
Authors:M Zalokar
Institution:(1) Centre de Génétique Moléculaire du C.N.R.S., and Université Pierre et Marie Curie, Paris VI, F-91190 Gif sur Yvette, (France)
Abstract:Summary Microinjection ofDrosophila eggs using the principle of thermal expansion is described. The eggs, mounted on cover slips with adhesive, are slightly dried to avoid the formation of exudate and then covered with fluorocarbon oil. Injections are made with a specially designed electrically controlled micropipette.
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