| 一株氢氧化细菌的生长条件及其对不同氮源利用的研究 |
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| 引用本文： | 陶虎春,谢勇,张丽娟,丁凌云,陈艺贞. 一株氢氧化细菌的生长条件及其对不同氮源利用的研究[J]. 北京大学学报(自然科学版), 2021, 57(4): 756-764. DOI: 10.13209/j.0479-8023.2021.059 |
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| 作者姓名： | 陶虎春 谢勇 张丽娟 丁凌云 陈艺贞 |
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| 作者单位： | 深圳市重金属污染控制与资源化重点实验室, 北京大学深圳研究生院环境与能源学院, 深圳 518055 |
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| 基金项目： | 国家自然科学基金(51679002, 51809004)、深圳市基础研究项目(JCYJ20180503182122539)和流域生态工程学学科建设资金(深发改〔2017〕542 号)资助 |
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| 摘 要： | 从污水处理厂活性污泥中成功地筛选出一株自养型氢氧化细菌(Hydrogen-oxidizing Bacterium,HOB),命名为Rhodoblastus sp.TH20.以模拟氨氮(NH4+-N)废水作为培养基,该菌株能够以H2为能源,CO2为碳源,其最适宜的生长条件为25℃、160 rpm和pH=7.0.当初始NH...
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| 关 键 词： | 氢氧化细菌 生长条件 氮源 微生物蛋白 |
| 收稿时间： | 2020-06-01 |
Growth Conditions of a Hydrogen-Oxidizing Bacterium and Its Utilization of Different Nitrogen Sources |
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| TAO Huchun,XIE Yong,ZHANG Lijuan,DING Lingyun,CHEN Yizhen. Growth Conditions of a Hydrogen-Oxidizing Bacterium and Its Utilization of Different Nitrogen Sources[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2021, 57(4): 756-764. DOI: 10.13209/j.0479-8023.2021.059 |
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| Authors: | TAO Huchun XIE Yong ZHANG Lijuan DING Lingyun CHEN Yizhen |
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| Affiliation: | Shenzhen Key Laboratory for Heavy Metal Remediation and Reclamation, School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055 |
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| Abstract: | An autotrophic hydrogen-oxidizing bacterium, named Rhodoblastus sp. TH20, was successfully enriched and isolated from municipal activated sludge in a domestic wastewater treatment plant. With simulated ammonium-containing wastewater as culture medium, the strain could utilize H2 and CO2 as energy and carbon sources respectively and the growth conditions were optimized at 25℃, 160 rpm and pH=7.0. At initial concentration of 100 mg/L, efficient ammonium removal (>99%) was achieved within 72 hours. Up to 77.8% of ammonium was assimilated to organic nitrogen and stored intracellularly, while the rest ammonium was transformed to gaseous N2. These results indicated that Rhodoblastus sp. TH20 was able to efficiently convert ammonium into microbial protein, thereby providing a sustainable pathway of wastewater treatment. |
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| Keywords: | hydrogen-oxidizing bacteria growth condition nitrogen source microbial protein |
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