2,4-二硝基甲苯磺酸盐降解菌株的分离、鉴定及降解特性研究

针对TNT红水污染土壤生物修复菌种资源匮乏问题,从复合菌群中分离纯化出一株2,4-二硝基甲苯磺酸盐(DNTS)降解菌株X2,经生理生化分析及16S rDNA鉴定,该菌株属于鞘氨醇单胞菌属.进一步研究环境因素对菌株X2生长的影响,并探究X2对2,4-二硝基甲苯-3-磺酸盐(2,4-DNT-3-SO3?)和2,4-二硝基甲...

Isolation and Identification of a 2,4-Dinitrotosylate Degrading Strain and Its Degradation Characteristics

Due to the shortage of bacteria resources for bioremediation of TNT red water polluted soil, a 2,4-dinitrotoluene sulfonates degrading strain X2 was isolated from a commercial microbial consortium. Based on the 16S rDNA sequence analysis and the physicbiochemical characteristics, strain X2 was identified as Sphingobium sp.. The growth experiments were carried out to optimize the growth condition of X2. The biodegradation mechanisms of 2,4-DNT-3-SO₃^- and 2,4-DNT-5-SO₃^- by X2 and its biodegradation ability towards other niro-aromatic explosives were investigated. The results showed that the optimum growing conditions for X2 were 30^oC, pH=7 and 1% salinity. The removal efficiencies of 2,4-DNT-3-SO₃^- and 2,4-DNT-5-SO₃^- reached 100% after 12 and 3 days, respectively. Liquid chromatography-mass spectrometry analysis showed that the nitro groups in dinitrotoluene sulfonates were transformed to amino groups. Sphingobium sp. X2 has broad-spectrum degradation characteristics. It can degrade not only dinitrotoluene sulfonates, but also other nitroaromatic pollutants like 2,4,6-trinitrotoluene, dinitrotoluene and mononitrotoluene, providing possibilities for TNT red water bioremediation.