| Phylogenetic identification and microbial diversity in snow of the summit （8201 m） of Cho Oyu Mountain,Tibet |
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| 作者姓名： | TONG XiaoMei ;CHEN Fang ;YU Jun ;HUA Sang ;ASAN Ciren ;LUOSANG JiangBai ;WANG Wei ;YU Liang ;ZHENG XiaoGuang ;WANG Jian |
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| 作者单位： | [1]Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China; [2]Beijing Genomics Institute, Shenzhen, Shenzhen 518083, China; [3]Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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| 基金项目： | Supported by the Knowledge Innovation Project of Chinese Academy of Sciences (Grant No. KSCX2-SW-331) |
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| 摘 要： | The bacterial diversity and abundance in snow of the summit （8201 m） of Cho Oyu mountain, Tibet, were analyzed by 16S rRNA gene sequencing followed by scanning electronic microscopy analysis. Most of bacteria were found to be of spherical or oval shape （〉95%）. Bacterial 16S rDNA sequences were classified into 5 genera （Caulobacter, Ralstonia, Cupriavidus, Pelomonas and Pseudomonas）. Gammaproteobacteria were the most abundant （91.25%） among the library that consists of 594 clones. The sequences found in this study are highly similar to those previously retrieved from other cold environments, such as ice core, sea ice, permafrost and snow. The results showed that the cold and barren environments strongly influence the survival of bacteria. The high similarity among sequences retrieved from snow sample and other places, such as ocean, soil and water, suggested that the bacteria in snow, soil and water environments have the same origin.
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| 关 键 词： | 雪化干扰 16S rRNA 基因表达 微生物 |
| 收稿时间： | 2008-05-05 |
| 修稿时间： | 2008-06-05 |
Phylogenetic identification and microbial diversity in snow of the summit (8201 m) of Cho Oyu Mountain, Tibet |
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| TONG XiaoMei,;CHEN Fang,;YU Jun,;HUA Sang,;ASAN Ciren,;LUOSANG JiangBai,;WANG Wei,;YU Liang,;ZHENG XiaoGuang,;WANG Jian.Phylogenetic identification and microbial diversity in snow of the summit (8201 m) of Cho Oyu Mountain, Tibet[J].Chinese Science Bulletin,2008,53(21):3317-3323. |
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| Authors: | XiaoMei Tong Fang Chen Jun Yu Sang Hua Ciren Asan JiangBai Luosang Wei Wang Liang Yu XiaoGuang Zheng Jian Wang |
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| Institution: | (1) Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 101300, China;(2) Beijing Genomics Institute, Shenzhen, Shenzhen, 518083, China;(3) Graduate University of Chinese Academy of Sciences, Beijing, 100049, China |
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| Abstract: | The bacterial diversity and abundance in snow of the summit (8201 m) of Cho Oyu mountain, Tibet, were analyzed by 16S rRNA
gene sequencing followed by scanning electronic microscopy analysis. Most of bacteria were found to be of spherical or oval
shape (>95%). Bacterial 16S rDNA sequences were classified into 5 genera (Caulobacter, Ralstonia, Cupriavidus, Pelomonas and Pseudomonas). Gammaproteobacteria were the most abundant (91.25%) among the library that consists of 594 clones. The sequences found
in this study are highly similar to those previously retrieved from other cold environments, such as ice core, sea ice, permafrost
and snow. The results showed that the cold and barren environments strongly influence the survival of bacteria. The high similarity
among sequences retrieved from snow sample and other places, such as ocean, soil and water, suggested that the bacteria in
snow, soil and water environments have the same origin.
Contributed equally to this work
Supported by the Knowledge Innovation Project of Chinese Academy of Sciences (Grant No. KSCX2-SW-331). |
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| Keywords: | Cho Oyu snow 16S rRNA gene phylogenetic tree |
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