| Cloning of lea cDNA
fragment of carrot (Daucus carota L.) and analysis
of its expression features |
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| 作者姓名： | . |
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| 作者单位： | QI Mei,HUANG Meijuan(Institute of Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China);CHEN Fan,WU Naihu(College of Life Sciences, Peking University, Beijing 100871, China)
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| 摘 要： |
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Cloning of lea cDNA fragment of carrot (Daucus carota L.) and analysis of its expression features |
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| ..Cloning of lea cDNA
fragment of carrot (Daucus carota L.) and analysis
of its expression features[J].Chinese Science Bulletin,2000,45(2):SDc226-515. |
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| Authors: | QI Mei CHEN Fan HUANG Meijuan WU Naihu |
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| Abstract: | Addition of concentrated sucrose to MS culture arrests the development of carrot somatic embryo at the stage of cotyledon embryo and, with the sucrose concentration restored to normal level, the embryo thus arrested is reactivated into post-embryonic development. Using the method of RT-PCR, the cDNA fragment of a new member of the Dc3 family of lea has been obtained from carrot somatic embryo under regulated state. As revealed by Northern blotting, strong expression has been observed in carrot somatic embryo under regulated state but the expression was much reduced 12 h after deregulation, and nearly disappeared 24 h after. Based on this finding as well as results of related studies, it is surmised that changing the sucrose concentration in culture enabled carrot somatic embryo under suspension culture to undergo a specific course of development which is comparable to the dormancy-germination process of seeds. |
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| Keywords: | carrot somatic embryo regulated system lea expression control |
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