正交试验优选木通中木通酸和木通皂苷Stc的提取工艺

研究木通中2个共有成分木通酸和木通皂苷Stc的提取工艺.采用超声提取,以溶剂种类、提取时间、溶剂用量为影响因素,各取3个水平,采用L9(34)正交表,以木通酸和木通皂苷Stc为考察指标,利用反相高效液相色谱法测定其含量,并对检测结果进行直观分析和方差分析.经正交试验优选的提取工艺:以体积分数为80%的乙醇作溶剂,采用超声提取1.5 h,溶剂用量为药材的12.5倍.对优选的提取工艺进行方法学考察,各项考察结果均符合要求.该提取工艺切实可行,为进一步对不同来源木通药材中木通酸和木通皂苷Stc的含量测定奠定理论基础.

Optimization of the Extraction Technics for Quinatic Acid and Akeboside Stc from Mutong by Orthogonal Test

Studies the optimization technique in extracting the quinatic acid and akeboside Stc in Mutong from Akebia.Ultrasonic extraction was applied.The variety of solvent,extraction time and reagent proportion were selected as the factors and were numbered in three levels respectively.L9(34) orthogonal test was adopted.Using the quinatic acid and akeboside Stc as indicator,measuring the content with reversed phase high-performance liquid chromatography(RP-HPLC) and variance analysis as well as directly analyzing on the measurement were carried out.The optimal condition of the orthogonal test was as follows: the solid dissolved in 80% ethanol,ultrasonic extraction lasted for 1.5 hours,and 12.5 times of solvent was needed.The result of methodological investigation for the optimum technique passed the muster.The technique proved to be practical,which laid theoretical foundation for measuring the quinatic acid and akeboside Stc in different kinds of Mutong from Akebia.