| LL-37/hCAP-18基因克隆及其真核表达载体的构建 |
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| 引用本文： | 杨应华,符碧.LL-37/hCAP-18基因克隆及其真核表达载体的构建[J].海南师范大学学报(自然科学版),2004,17(3):265-269. |
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| 作者姓名： | 杨应华 符碧 |
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| 作者单位： | 海南师范学院,生物系,海南,海口,571158 |
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| 基金项目： | 海南师范学院博士科研启动基金资助项目 |
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| 摘 要： | LL-37 hCAP-18是一种重要的多功能免疫分子.为探讨hCAP-18在基因治疗中的可行性,应用RT-PCR技术从人白血病细胞系J6-1细胞总RNA中成功扩增出560bp的hCAP-18基因编码区cDNA序列,构建了hCAP-18重组表达质粒phCAP-18.采用脂质体法将质粒phCAP-18瞬时转染COS-7细胞,Westernblot证实,hCAP-18能在COS-7细胞中表达.
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| 关 键 词： | LL-37hCAP-18 cDNA克隆 真核表达 |
| 文章编号： | 1671-8747(2004)03-0265-05 |
| 修稿时间： | 2004年3月22日 |
Cloning and construction of the eukaryotic expression vector of human antimicrobial peptide LL-37/hCAP-18 |
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| YANG Ying-hua,FU Bi.Cloning and construction of the eukaryotic expression vector of human antimicrobial peptide LL-37/hCAP-18[J].Journal of Hainan Normal University:Natural Science,2004,17(3):265-269. |
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| Authors: | YANG Ying-hua FU Bi |
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| Abstract: | The human antimicrobial peptide LL-37/hCAP-18 is a multifunctional modulator of innate immune responses.To clone the hCAP-18 gene and to construct the eukaryotic expression vector,total RNA was extracted from J6-1 cells,then the hCAP-18 cDNA was amplified by RT-PCR and cloned into the eukaryotic expression vector pTARGET.Results of sequencing showed that the orientation of the ligations and the reading frame were correct.The resombimant plasmid was transfected into COS-7 cells,and Western blotting indicated that the recombinat of vector could express LL-37/hCAP-18 protein in COS-7 cells. |
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| Keywords: | LL-37/hCAP-18 cDNA cloning eukaryotic expression |
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