| 天蚕抗菌肽B基因在毕赤酵母中多拷贝表达载体的构建 |
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| 引用本文： | 扈进冬,吴远征,张广志,杨合同.天蚕抗菌肽B基因在毕赤酵母中多拷贝表达载体的构建[J].山东科学,2008,21(5):21-24. |
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| 作者姓名： | 扈进冬 吴远征 张广志 杨合同 |
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| 作者单位： | 1. 山东省科学院中日友好生物技术研究中心,山东省应用微生物重点实验室,山东,济南,250014
2. 山东省科学院中日友好生物技术研究中心,山东省应用微生物重点实验室,山东,济南,250014;山东省科学院生物所,山东,济南,250014;山东理工大学生命科学院,山东,淄博,255049 |
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| 基金项目： | 国家高技术研究发展计划(863计划)现代农业技术领域重大项目
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| 摘 要： | 根据Bgl Ⅱ和BamHⅠ同尾酶的特点，通过对pPIC9K／CB进行改造，获得了不含BamHⅠ位点的CecropinB表达框，并在此基础上构建了含三拷贝CecropinB表达框的毕赤酵母表达载体，为CecropinB的进一步开发与应用打下了基础。
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| 关 键 词： | 天蚕素B 毕赤酵母 多拷贝 载体构建 |
The Construction of Repeats Copy Expression Vector of Cecropin B in Pichia pastoris |
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| HUO Jin-dong,WU Yuan-zheng,ZHANG Guang-zhi,YANG He-tong.The Construction of Repeats Copy Expression Vector of Cecropin B in Pichia pastoris[J].Shandong Science,2008,21(5):21-24. |
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| Authors: | HUO Jin-dong WU Yuan-zheng ZHANG Guang-zhi YANG He-tong |
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| Institution: | ( Biotechnology Research Center, Shandong Acadeny of Sciences ; Key Laboratory for Applied Microbiology of Shandong Province, Jinan 250014, China ; Institute of Biology, Shandong Academy of Sciences, Jinan 250014, China ; School of Life Sciences, Shandong University of Technology, Zibo 255049, China) |
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| Abstract: | The new expression box of Cecropin B without the restriction to the site of BamH I was obtained by the transformation of pPIC9K/CB, which was based on the characteristics of isocaudamers of BglII and BamH I. The expression vector of Pichia pastoris containing three copies of Cecropin B was therefore constructed, which laid the foundations for farther development and applications of Cecropin B. |
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| Keywords: | Cecmpin B Pichia pastoris repeats copy construction of expression vector |
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