| OFFGEL和LC-MS联用分析SH-SY5Y 细胞分泌蛋白质组 |
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| 引用本文： | 张永谦,来成俊,李琰,卡里姆,邓玉林. OFFGEL和LC-MS联用分析SH-SY5Y 细胞分泌蛋白质组[J]. 北京理工大学学报, 2011, 31(10): 1252-1255 |
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| 作者姓名： | 张永谦 来成俊 李琰 卡里姆 邓玉林 |
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| 作者单位： | 1. 北京理工大学生命学院,北京,100081
2. 河北化工医药职业技术学院,河北,石家庄050026 |
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| 基金项目： | 北京理工大学研究生科技创新基金资助项目(AA200805) |
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| 摘 要： | 建立了游离胶分馏器(OFFGEL)和反相液相色谱质谱(LC-MS)联用分析SH-SY5Y细胞分泌蛋白质组的新方法.收集SH-SY5Y胞外蛋白用胰酶酶切成肽段后,用OFFGEL进行预分离并用LC-MS进行检测.用Mascot检索出88个蛋白,再通过生物信息学软件SignalP对Mascot检索的蛋白进行预测和筛选,共得到31种蛋白,用SingalP中的神经网络法和隐马可夫模型对其中的CERU_HUMAN进行分析,发现该蛋白是分泌蛋白,且信号肽的剪切位点在第19和第20氨基酸之间,软件SingalP提升了鉴定分泌蛋白的可靠性.
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| 关 键 词： | 分泌蛋白组 游离胶分馏器 液相色谱质谱 生物信息学 |
| 收稿时间： | 2010-12-15 |
Secretome Analysis of Human SH-SY5Y Cells Using OFFGEL and Capillary Liquid Chromatography with Electrospray Tandem Mass Spectrometry |
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| ZHANG Yong-qian,LAI Cheng-jun,LI Yan,Kaleemullah and DENG Yu-lin. Secretome Analysis of Human SH-SY5Y Cells Using OFFGEL and Capillary Liquid Chromatography with Electrospray Tandem Mass Spectrometry[J]. Journal of Beijing Institute of Technology(Natural Science Edition), 2011, 31(10): 1252-1255 |
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| Authors: | ZHANG Yong-qian LAI Cheng-jun LI Yan Kaleemullah DENG Yu-lin |
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| Affiliation: | ZHANG Yong-qian1,LAI Cheng-jun1,LI Yan2,Kaleemullah1,DENG Yu-lin1(1.School of Life Science,Beijing Institute of Technology,Beijing 100081,China,2.Hebei Vocational and Technical Institute of Chemistry and Medicine,Shijiazhuang,Hebei 050026,China) |
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| Abstract: | A new assay has been developed to analyze the secretome of human SH-SY5Y by OFFGEL and LC-MS combined with the software of bioinformatics.The proteins collected from the extracellular SH-SY5Y mediums have been digested and fractionated by the OFFGEL,and then different fractions were performed by reverse phase liquid chromatography with electrospray tandem mass spectrometry.The 88 potential secretory proteins from Mascot results were predicted and screened by the bioinformatics software called SignalP.Only 3... |
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| Keywords: | secretome OFFGEL LC-MS bioinformatics |
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