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禽畜粪肥对农田蔬菜中抗生素抗性内生菌的影响
引用本文:王瑞飞,郭玉晖,俞宁,杨清香.禽畜粪肥对农田蔬菜中抗生素抗性内生菌的影响[J].河南师范大学学报(自然科学版),2016(4):106-111.
作者姓名:王瑞飞  郭玉晖  俞宁  杨清香
作者单位:河南师范大学生命科学学院;河南师范大学资源微生物与功能分子河南省高校重点实验室培育基地;新乡市环境保护监测站
基金项目:国家自然科学基金(21477035;21277041);教育部博士点基金项目(20134104110006)
摘    要:选取常食农田茎叶类蔬菜——上海青和芹菜为研究对象,探讨禽畜粪肥对蔬菜抗生素抗性内生菌的影响.结果表明:施加粪肥的农田上海青及芹菜样品的根、茎、叶中抗阿莫西林、头孢氨苄、四环素的单抗性内生细菌的数量均比未施加粪肥组高,它们的相对百分比含量比未施加组高出9~19倍.其中,从施加禽畜粪肥上海青中分离获得的多重抗生素抗性细菌-嗜麦芽寡养单胞菌,可能引起多种人类疾病,尤其值得关注.

关 键 词:抗生素  蔬菜  抗生素性内生细菌  禽畜粪便

Effect of Livestock Manure on Antibiotic-Resistant Endophytic Bacteria in Vegetables
WANG Ruifei;GUO Yuhui;YU Ning;YANG Qingxiang.Effect of Livestock Manure on Antibiotic-Resistant Endophytic Bacteria in Vegetables[J].Journal of Henan Normal University(Natural Science),2016(4):106-111.
Authors:WANG Ruifei;GUO Yuhui;YU Ning;YANG Qingxiang
Institution:WANG Ruifei;GUO Yuhui;YU Ning;YANG Qingxiang;College of Life Sciences,Henan Normal University;Key Laboratory for Microorganisms and Functional Molecules,University of Henan Province,Henan Normal University;Monitoring Station of Environmental Protection in Xinxiang City;
Abstract:In this paper,we investigated the effects of application of livestock manure on antibiotic-resistant endophytic bacteria in pakchoi and celery which are often consumed as popular stems and leafy vegetables.The result indicated that the count and proportion of endophytic bacteria resistant to Amoxicilln trihydrade,Cephalexin monohydrate or Tetracyclin Hydrachloride in root,stem and leaf of manure-fertililzed pakchoi and celery was 9-19 folds higher than that in manure-free pakchoi and celery.Notably,Stenotrophomonas maltophilia of these bacteria is an opportunistic pathogen to humans and can cause various disease infections.
Keywords:antibiotic  vegetable  antibiotic-resistant endophytic bacteria  livestock manure
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