可降解聚醚酯弹性体PTCG的合成及初步生物学评价

用熔融缩聚法合成聚对苯二甲酸丁二醇酯-co-聚对苯二甲酸环己烷二甲醇酯-b-聚乙二醇(PTCG)聚醚酯弹性体.按照ISO 10993标准,采用L929小鼠成纤细胞对其进行体外细胞毒性测试;以狗的血管平滑肌细胞 (SMCs)为模板细胞,测试细胞在材料表面的贴附性能.采用旋转成型/粒子洗出法制备三维血管支架,接种SMCs后在生物反应器上进行动态培养,探索PTCG作为组织工程血管支架材料的可行性.结果表明,合成的PTCG聚醚酯弹性体无细胞毒性;SMCs在PTCG薄膜表面贴附生长良好;所制备支架的孔径、孔隙率和力学性能等满足组织工程血管支架的要求;体外培养3 d后,大量SMCs长入血管支架并开始分化.

Synthesis of PTCG Poly(Ether Ester) Elastomer and a Preliminary Biocompatible Evaluation

A series of poly(butylene terephthalate)-co-poly(cyclohexylene dimethyl terephthalate)-b-poly(ethylene glycol) (PTCG) copolymers were synthesized by a two-step polycondensation. The in vitro cytotoxicity test of PTCG was carried out according to ISO10993 standard. Dog's vascular smooth muscle cells (SMCs) were used as model cells to test the cell affinity on PTCG films. 3D vascular scaffolds were fabricated by a spin-molding/particle leaching technique. SMCs were inoculated on the scaffolds and then cultured in a bioreactor. The copolymer extracts showed no cytotoxic reactivity in the in vitro cytotoxity test. SMCs adhered to the PTCG films and grew well. The scaffolds appeared to meet the demands of tissue engineering on vascular scaffolds as to the porosity, pore size and mechanical properties. After the 3 d culture, SMCs grew into the scaffolds and began to proliferate.