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Stabilisierung der oxydativen Phosphorylierung isolierter Mitochondrien durch artspezifische und artunspezifische Serumproteine
Authors:G Laudahn
Institution:(1) Hauptlabor des Städtischen Krankenhauses Berlin-Wilmersdorf, Germany
Abstract:Summary Addition of 3 mg bovine serum albumin/mg mitochondrial-N enhances and stabilizes the phosphorylation capacity of isolated beef liver mitochondria. Albumin reduces the fluctation of the resulting P/O-rates and brings about a better reproducibility of the entire method. The same effect is produced by addition of bovine gamma-globulin in half the concentration of albumin, while the effect of heterologous proteins (e. g. human albumin) is inferior.
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