Stabilisierung der oxydativen Phosphorylierung isolierter Mitochondrien durch artspezifische und artunspezifische Serumproteine |
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Authors: | G Laudahn |
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Institution: | (1) Hauptlabor des Städtischen Krankenhauses Berlin-Wilmersdorf, Germany |
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Abstract: | Summary Addition of 3 mg bovine serum albumin/mg mitochondrial-N enhances and stabilizes the phosphorylation capacity of isolated beef liver mitochondria. Albumin reduces the fluctation of the resulting P/O-rates and brings about a better reproducibility of the entire method. The same effect is produced by addition of bovine -globulin in half the concentration of albumin, while the effect of heterologous proteins (e. g. human albumin) is inferior. |
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