| 响应面法优化茶叶多糖提取条件的研究 |
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| 引用本文： | 谢亮亮,蔡为荣.响应面法优化茶叶多糖提取条件的研究[J].安徽工程科技学院学报,2013(4):8-11. |
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| 作者姓名： | 谢亮亮 蔡为荣 |
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| 作者单位： | 安徽工程大学生物与化学工程学院; |
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| 基金项目： | 安徽省国际合作基金资助项目(10080703035) |
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| 摘 要： | 采用水提法从茶叶中提取粗多糖，采用响应面Box-Behnken实验设计方法对提取时间、液固比和提取温度进行优化，从而获得最佳的多糖提取工艺．采用多元回归分析对实验数据进行拟合对并其进行统计学方差分析，得最佳提取条件为：提取时间106min，液固比30，提取温度64℃．在此优化条件下，粗多糖得率为13．26％，与预测值13．14％非常接近．
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| 关 键 词： | 响应面 提取时间 液固比 提取温度 |
Optimization of extraction of polysaccharides from the leaves of camellia sinensis using respond surface method |
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| XIE Liang-liang,CAI Wei-rong.Optimization of extraction of polysaccharides from the leaves of camellia sinensis using respond surface method[J].Journal of Anhui University of Technology and Science,2013(4):8-11. |
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| Authors: | XIE Liang-liang CAI Wei-rong |
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| Institution: | (Coll. of Bioeh. Engn. ,Anhui Polytec＇hnie University, Wuhu 241000, China) |
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| Abstract: | The crude polysaccharides were extracted from the leaves of Camellia sinensis using water ex- traction method. Respond surface method based on Box-Behnken design was employed to obtain the best polysaccharides extraction process parameters.extraction time, water to the raw material ratio and ex- traction temperature. The experimental data were fitted using multiple regression analysis and also ana- lyzed by appropriate statistical method. The optimum extraction conditions were as follows： extraction time of 106 rain, water to the raw material ratio of 30 and extraction temperature of 64 ℃. Under these conditions, the exnerimental yield was 13.26% ,close the expected value of 13.14%. |
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| Keywords: | respond surface method extraction time water to the raw material ratio extraction tenaper-ature |
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