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Identification of proteins in human prostate tumor material by two-dimensional gel electrophoresis and mass spectrometry |
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| Authors: | A. A. Alaiya M. Oppermann? J. Langridge? U. Roblick L. Egevad S. Br?ndstedt M. Hellstr?m S. Linder T. Bergman H. J?rnvall G. Auer |
| Affiliation: | (1) Department of Oncology and Pathology, Karolinska Institutet and Hospital, SE-17176 Stockholm (Sweden), Fax +46 8 331696, e-mail: gert.auer@cck.ki.se , SE;(2) Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm (Sweden) , SE;(3) Micromass UK Ltd, Manchester (UK) , GB;(4) Department of Urology, Karolinska Institutet and Hospital, Stockholm (Sweden) , SE |
| Abstract: | Protein patterns in cells collected from benign prostatic tissues and prostate carcinomas were analyzed using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Polypeptide expression was evaluated by computer-assisted image analysis (PDQUEST). Proteins expressed by prostate tumors were identified via in-gel digestion and subsequent matrix-assisted laser desorption/ionization mass spectrometry. In addition to cytoskeletal and mitochondrial proteins, a 40-kDa protein was identified as prostatic acid phosphatase (PAP). PAP expression decreased approximately twofold between benign and malignant tissue. Increased expression of heat shock protein 70 and decreased expression of tropomyosin 1 were also observed in the malignant tissue. The analysis of prostate material by two-dimensional gel electrophoresis and mass spectrometry shows that particular proteins are of interest as markers of disease. Received 18 December 2000; accepted 4 January 2001 |
| Keywords: | . Prostatic carcinoma benign prostatic tissue two-dimensional gel electrophoresis mass spectrometry. |
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