| 三种罗布麻干燥叶片基因组DNA的提取方法 |
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| 引用本文： | 张卫明,王曼丽,彭雪梅,薛华杰,陆长梅.三种罗布麻干燥叶片基因组DNA的提取方法[J].南京师大学报,2007,30(1):102-105. |
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| 作者姓名： | 张卫明 王曼丽 彭雪梅 薛华杰 陆长梅 |
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| 作者单位： | [1]南京师范大学生命科学学院,江苏南京210097 [2]南京野生植物综合利用研究院,江苏南京210042 |
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| 基金项目： | 科技攻关基金
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江苏省高新技术项目 |
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| 摘 要： | 采用Qiagen的植物基因组DNA提取试剂盒并加以改进,首次从不同季节采收、不同干燥方法保存的三种罗布麻(罗布红麻、大叶白麻和白麻)干燥叶片中提取出基因组DNA.提取的基因组TE溶解液的OD260/OD280 值多在1.7～1.9范围内,电泳呈现单条带,核基因组的ITS片段、叶绿体基因组的trnL内含子和trnL-F非编码区片段的PCR扩增条带清晰并能进行DNA测序,这表明基因组DNA质量较好,可以满足后续分子生物学实验的要求.本研究为罗布麻等中药材的进一步分子生物学研究提供了必要的技术基础.
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| 关 键 词： | 罗布麻叶 DNA提取 PCR 罗布麻 叶绿体基因组 提取 干燥方法 Leaves Genomic DNA 技术基础 生物学研究 中药材 生物学实验 分子 质量 测序 扩增条带 非编码区 内含子 核基因组 单条 电泳 范围 |
| 文章编号： | 1001-4616（2007）01-0102-04 |
| 收稿时间： | 2006-09-26 |
| 修稿时间： | 2006-10-20 |
Isolation of Genomic DNA From Dried Leaves of Three Kinds of Luobuma |
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| Zhang Weiming,Wang Manli,Peng Xuemei,Xue Huajie,Lu Changmei.Isolation of Genomic DNA From Dried Leaves of Three Kinds of Luobuma[J].Journal of Nanjing Normal University(Natural Science Edition),2007,30(1):102-105. |
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| Authors: | Zhang Weiming Wang Manli Peng Xuemei Xue Huajie Lu Changmei |
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| Institution: | 1. School of Life Science, Nanjing Normal University, Nanjing 210097, China;2. Nanjing Institute for Comprehensive Utilization of Wild Plant, Nanjing 210042, China |
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| Abstract: | Genomic DNA was first isolated from dried leaves of Luobuma by the modified method of the Qiagen DNeasy plant mini kit. Results showed that by this method genomic DNA could be isolated from Luobuma leaves of three species being collected at different seasons and being preserved by different methods. The OD260/OD280 ratios of the isolated DNA were in the range of 1.7- 1.9, and only one band presented in agarose gels ; clear PCR bands of ITS rDNA and tmL intron, tmL - F non - coding cpDNA could be sequenced. All the above revealed that the isolated DNA was complete and it could fulfill further demand for molecular biological experiment. |
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| Keywords: | leaves of Luobuma DNA isolation PCR |
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