| 医用脲酶菌的固定化比较研究 |
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| 引用本文： | 高虹,陈明,郑津辉.医用脲酶菌的固定化比较研究[J].天津师范大学学报(自然科学版),2011,31(1):66-70. |
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| 作者姓名： | 高虹 陈明 郑津辉 |
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| 作者单位： | 天津师范大学生命科学学院,天津,300387 |
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| 基金项目： | 天津师范大学青年基金资助项目 |
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| 摘 要： | 以聚乙烯醇为载体,分别通过化学法与物理法对医用脲酶菌进行包埋,并比较了2种方法所制备微球的性能及血清尿素清除活性.化学法采用硼酸交联,当调节硼酸pH至6.5时所得固定化微球比未调硼酸pH(4.0)的微球有较高的机械强度及尿素吸收活性;物理法采用冷冻交联,所得微球粒径均一,机械强度较好,冷冻交联微球吸收尿素的活性与未调p...
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| 关 键 词： | 脲酶菌 尿毒症 口服微球 固定化 聚乙烯醇 |
Comparative study on immobilizition of urease-producing bacteria used in therapy |
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| GAO Hong,CHEN Ming,ZHENG Jinhui.Comparative study on immobilizition of urease-producing bacteria used in therapy[J].Journal of Tianjin Normal University(Natural Science Edition),2011,31(1):66-70. |
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| Authors: | GAO Hong CHEN Ming ZHENG Jinhui |
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| Institution: | GAO Hong,CHEN Ming,ZHENG Jinhui College of Life Science,Tianjin Normal University,Tianjin 300387,China |
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| Abstract: | Urease-producing bacteria used in therapy were entrapped through chemical and physical methods by use of polyvinyl alcohol as the carrier.The properties and serum urea clearance activities of two sorts of microspheres were compared.Microsphere of chemical cross-linking with boric acid pH 6.5 had higher mechanical strength and urea clearance activity than that with boric acid pH 4.0.The freeze-thawing method was employed for physical cross-linking.Microsphere of physical cross-linking had a uniform diameter ... |
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| Keywords: | urease-producing bacteria uremia oral microsphere immobilization PVA |
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