Hela细胞中突变PSF蛋白对细胞增殖迁移及可变剪接的影响

PSF作为真核细胞中的抑癌蛋白,在Hela细胞发生基因突变导致其蛋白功能改变.为了阐明突变体PSF蛋白在肿瘤细胞的作用,本文分别采用siRNA干扰、细胞生长曲线、细胞划痕等实验检测muPSF对Hela细胞增殖及迁移的影响,半定量PCR实验分析PSF调控的下游靶基因的可变剪切情况.研究结果显示,当通过siRNA干扰muPSF的表达后Hela细胞的增殖迁移能力下降,高表达突变体PSF可增强Hela细胞的增殖与迁移,半定量PCR实验分析PSF下游调控基因显示muPSF可以改变增殖和迁移相关基因的可变剪接形式.因此,我们的实验证明,Hela细胞中muPSF通过调控下游靶基因的可变剪切影响Hela细胞的增殖和迁移能力.

Mutant of PSF effects on the prolification, migration and alternative splicing

PSF is an important tumor suppressor protein in eukaryotic cells. In Hela cells, PSF gene is mutant and leads to transform of its function. In order to clarify the function of PSF mutant (muPSF) in Hela cells, muPSF was knocked down by designed siRNA. Through the cell growth, wound scratch assay and detecting alternative splicing (AS) of downstream genes by semiquantitative PCR. The results showed that RNAi of muPSF expression could down regulate migration and proliferation of Hela cells, and rescue of muPSF had its ability to enhance the migration and proliferation. The study confirmed that muPSF, in Hela cells, regulated the migration and proliferation by AS of downstream genes.