| 产细菌素乳酸菌的筛选及其纯化和稳定性研究 |
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| 引用本文： | 陈沛,王雪青,阮海华.产细菌素乳酸菌的筛选及其纯化和稳定性研究[J].天津师范大学学报(自然科学版),2011,31(2):82-85. |
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| 作者姓名： | 陈沛 王雪青 阮海华 |
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| 作者单位： | 1. 天津商业大学,天津市食品与生物技术重点实验室,天津,300134
2. 天津商业大学,物技术与食品科学学院,天津,300134 |
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| 基金项目： | 天津市科委应用基础重点资助项目，国家科技部资助项目 |
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| 摘 要： | 以大肠杆菌、金黄色葡萄球菌、藤黄微球菌、单增李斯特菌、枯草芽孢杆菌、志贺氏菌、沙门氏菌为指示菌,从30个健康婴儿的粪便中筛选得到1株具有广谱抑菌作用的菌株,经鉴定为乳酸乳球菌(编号F-6-B).该菌株的培养条件为:MRS培养基起始pH 6.0,30℃静置培养48 h.将其发酵液经硫酸铵沉淀,Hitrap SPHP(GE...
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| 关 键 词： | 细菌素 乳酸菌 抑菌作用 |
Screening bacteriocin-producing lactic acid bacteria and purification of bacteriocin and its stability |
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| CHEN Pei,WANG Xueqing,RUAN Haihua.Screening bacteriocin-producing lactic acid bacteria and purification of bacteriocin and its stability[J].Journal of Tianjin Normal University(Natural Science Edition),2011,31(2):82-85. |
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| Authors: | CHEN Pei WANG Xueqing RUAN Haihua |
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| Institution: | a.Tianjin Key Laboratory of Food and Biotechnogy,b.College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China |
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| Abstract: | One strain of bacteriocin-producing bacterium was isolated from feces of 30 healthy infants,using Escherichia coli,salmonellas Micrococcus Luteus Listera,bacillus subtilis,shigella,Staphylococcus aureus as indicate germs,and was identified as Lactococcus schleifer(number F-6-B).The bacteriocin was obtained from the fermentation broth of culture by ammonium sulfate precipitation and ion-exchange chromatography of Hitrap SPHP(GE).The molecular weight of the bacteriocin was 3 300-5 800 that determined by Tricine-SDS-PAGE.Treated with acid,heat and enzyme,the bacteriocin still had significant effects on bacteriostasis,exhibiting its better thermostability,a broad-spectrum pH range of activity and poor sensitivity to trypsin. |
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| Keywords: | bacteriocin lactic acid bacteria antibacterial ability |
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