Co2+对红霉素生物合成过程的影响

研究了红霉素生物合成过程中糖代谢相关的关键酶:己糖激酶、6-磷酸葡萄糖脱氢酶、磷酸果糖激酶和异柠檬酸脱氢酶,以及合成红霉内酯环前体甲基丙二酰CoA相关的酶:甲基丙二酰CoA异构酶和甲基丙二酰CoA羧基转移酶的时序变化,并测定了过程中有机酸的含量变化。实验表明:Co2 能大幅提高红霉素效价。这可能与Co2 能明显提高甲基丙二酰CoA异构酶与甲基丙二酰CoA羧基转移酶的比活,并在一定程度上促进糖代谢有关。

Effect of Co2+ on the Erythromycin Biosynthesis

Erythromycin biosynthesis is a highly complicated process,which involves both primary metabolism and secondary metabolism.The activities of the key enzymes related to glucose metabolism such as glucose kinase,glucose-6-phosphate dehydrogenase,phosphofrutokinase and isocitrate dehydrogenase in Saccharopolyspora erythraea,and the activities of the enzymes involved in secondary metabolism,such as methylmalonyl-coenzyme A mutase,and methylmalonyl-coenzyme A transcaboxylase,were determined.Some organic acids in fermentation broth were also analyzed.Results show that Co~(2 ) increases erythromycin biosynthesis.It maybe owe to improvement of the specific activities of methylmalonyl-coenzyme A mutase and methylmalonyl-coenzyme A transcaboxylase by Co~(2 ).Meanwhile,it also increases the flux of glucose metabolism pathway.