| 基于全内反射荧光显微镜的单个心肌细胞内钙信号斑图的介观表征 |
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| 引用本文： | 白永强,朱星. 基于全内反射荧光显微镜的单个心肌细胞内钙信号斑图的介观表征[J]. 吉林大学学报(理学版), 2010, 48(4): 672-675 |
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| 作者姓名： | 白永强 朱星 |
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| 作者单位： | 1. 大庆石油学院 电子科学学院, 黑龙江 大庆 163318;2. 北京大学 物理学院人工微结构与介观物理国家重点实验室, 北京 100871 |
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| 基金项目： | 国家重点基础研究发展计划973项目基金，黑龙江省自然科学基金 |
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| 摘 要： | 利用基于近场光学原理组建的全内反射荧光显微镜研究单个大鼠心肌细胞内钙离子信号的斑图.结果表明:存在于盖玻片表面约100nm区域的隐失场可较好地照射活体细胞,并能清晰地显示胞内信号;心肌细胞内钙信号包括钙火花、钙靶波、钙平面波和钙螺旋波等形式,钙信号各形式间存在相互作用;在钙诱导钙释放机制作用下,心肌细胞具有较强的可激发特性.
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| 关 键 词： | 全内反射荧光显微镜 钙火花 钙螺旋波 钙诱导钙释放 可激发特性 |
| 收稿时间： | 2009-12-18 |
Mesoscopic Representation on Patterns of Ca2+ Signals in Single Heart Cell Based on Total Internal Reflection Fluorescence Microscope |
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| BAI Yong-qiang,ZHU Xing. Mesoscopic Representation on Patterns of Ca2+ Signals in Single Heart Cell Based on Total Internal Reflection Fluorescence Microscope[J]. Journal of Jilin University: Sci Ed, 2010, 48(4): 672-675 |
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| Authors: | BAI Yong-qiang ZHU Xing |
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| Affiliation: | 1. College of Electronic Science, Daqing Petroleum Institute, Daqing 163318, Heilongjiang Province, China|2. School of Physics, State Key Laboratory for Artificial Microstructure and Mesoscopic Physics, Peking University, Beijing 100871, China |
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| Abstract: | The signals of calcium ions in single rat heart cell were studied by means of a total internal reflection fluorescence microscope made by the theory of near-field optics. The results show that Ca2+ signals can be detected clearly under the illumination of evanescence which only penetrated cell about 100 nm. Ca2+ signals in single heart cell show Ca2+ sparks, Ca2+ target waves, Ca2+ plane waves and Ca2+ spiral waves, and these forms interact to each other. Further more, single heart cells show strong excitability under calcium|induced calcium release mechanism. |
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| Keywords: | total internal reflection fluorescence microscope (TIRFM) Ca2+ spark Ca2+ spiral wave calcium induced calcium release (CICR) excitability |
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