白芥子多糖的提取与含量测定

用水提醇沉法提取白芥子多糖,用苯酚—硫酸比色法测定多糖含量.采用单因素和正交实验L9（33）考察了提取温度、提取时间、料液比、浸提次数和粉末粗细对白芥子多糖提取率的影响,从而确定出最佳的提取方案,并测定白芥子粗多糖的含量.结果表明,最佳提取工艺为A3B2C3,即在温度98℃下,料液比为1∶25,水提取2 h;此外,单因素实验得,浸提2次,粉末为40目,加5倍量95%乙醇醇析为宜;测得白芥子中多糖含量为3.821%,平均回收率为97.86%,相对标准偏差为RSD%=0.19,（n=5）;各因素对白芥子多糖的提取率影响是不均等的,影响程度依次,为提取温度＆gt;料液比〉提取时间.

Extraction and Content Determination of Polysaccharide in Semen Brassicae Albae

To investigate the effects of various extraction conditions,on the polysaccharide extraction yield of semen Brassicae Albae,such as extraction temperature,extraction time,ratio of liquid to material and thickness of powder,one factor and orthogonal experiment were made to determine the best extraction method and to determine the content of polysaccharide in it.Method of water solution and alcohol sedimentation was used to extract polysaccharides and phenol-sulfuric acid colorimetry was applied to determine ...