| 拟南芥AtSuc3和AtSuc4基因的克隆及双价植物表达载体的构建 |
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| 引用本文： | 张彦伟,祝建波,沈海涛,王爱英,向本春.拟南芥AtSuc3和AtSuc4基因的克隆及双价植物表达载体的构建[J].石河子大学学报,2010,28(1):6-10. |
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| 作者姓名： | 张彦伟 祝建波 沈海涛 王爱英 向本春 |
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| 作者单位： | 石河子大学生命科学学院/石河子大学农业生物技术重点实验室; |
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| 摘 要： | 蔗糖转运蛋白在调节同化产物的分配过程中起重要作用。为了分析拟南芥蔗糖转运蛋白基因AtSuc3、At-Suc4的功能和协同作用,以哥伦比亚型拟南芥为材料,通过RT-PCR技术克隆了蔗糖转运蛋白基因(sucrose/H+cotransporters,SUCs)AtSuc3和AtSuc4的cDNA,利用甘薯贮藏蛋白基因(sporamin)的根部特异性启动子,构建了含有含有AtSuc3和AtSuc4 cDNA的单价植物表达载体pBI2301-q3-s3、pBI2301-q4-s4和双价植物表达载体pBI2301-q3-s3-q4-s4,为进一步分析该植物表达载体在调控库源关系中的作用以及在经济作物中的应用奠定基础。
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| 关 键 词： | 蔗糖转运蛋白 AtSuc3 AtSuc4 植物表达载体 构建 |
The cDNA Cloning of AtSuc3 and AtSuc4 Gene from Arabidopsis thaliana and Construction of Plant Expression Vector |
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| ZHANG Yanwei,ZHU Jianbo,SHEN Haitao,WANG Aiying,XIANG Benchun.The cDNA Cloning of AtSuc3 and AtSuc4 Gene from Arabidopsis thaliana and Construction of Plant Expression Vector[J].Journal of Shihezi University(Natural Science),2010,28(1):6-10. |
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| Authors: | ZHANG Yanwei ZHU Jianbo SHEN Haitao WANG Aiying XIANG Benchun |
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| Institution: | College of Life Science/Key Laboratory of Agricultural Biotechnology;Shihezi University;Shihezi 832003;China |
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| Abstract: | Sucrose transporter protein plays an important role in the regulation of distribution of assimilation products.In order to study the functions and synergies of Arabidopsis thaliana sucrose transporter gene AtSuc3 and AtSuc4,cDNA sequences of the AtSuc3 and AtSuc4 protein were cloned from the A.thaliana ecotypes Colombia(Col-0).Monovalent and bivalent plant expression vector with sweet potato storage protein gene driven by root-specific promoter were constructed and named pBI2301-q3-s3,pBI2301-q4-s4 and pBI2... |
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| Keywords: | sucrose transporter AtSuc3 AtSuc4 plant expressing vector construction] |
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