小麦耐盐愈伤组织内源脯氨酸含量和细胞学特征

以小麦幼穗和幼胚为外植体，诱导出愈伤组织，通过在培养基中逐渐增加选择剂（ＮａＣｌ：Ｎａ２ＳＯ４＝４：１）方法，获得耐０．８％至２．０％选择剂的耐盐愈伤组织，其内源脯氨酸含量显著高于相同盐渍条件下的对照组．培养基中选择剂浓度达到１．６％耐盐细胞仍能保持细胞及细胞器正常形态，液泡化程度低，细胞核大，有的细胞中出现二或二个以上核仁．在培养基中选择剂超过１．２％，绝大多数细胞处于坏死或类坏死状态．耐盐愈伤组织在培养基中选择剂不超过２．０％，生长良好，可以再生植株，其后代在０．３％ＮａＣｌ土壤中仍表现耐盐性．

CONTENTS OF FREE PROLINE AND CHARACTERS OF CELLS IN SALT-TOLERANT CALLI OF WHEAT

Calli of wheat established from the young inflorescences and immature embrys are used to select for salt tolerance and subcultured on solid medium containing gradually increasing concentrations of salt (NaCl: Na2SO4=4: 1) from 0. 8% to 2. 4% Compared with non - selected callus, the contents of free proline was increased singificantly. On medium containing 1. 6 % NaCl and Na2SO4, cells and their organelles in salt -tolerant calli had normal morphology and structure, in non -selected calli, the majority of cells turned towards dying. the tolerant regenerated plant had grown in non-salted solid and their progenies had grown in NaCl solid.