| 刺猬和三种游蛇科动物冬眠期和活动期乳酸脱氢酶同工酶的比较 |
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| 引用本文： | 任文华,杨光,等.刺猬和三种游蛇科动物冬眠期和活动期乳酸脱氢酶同工酶的比较[J].南京师大学报,2002,25(1):20-23. |
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| 作者姓名： | 任文华 杨光 |
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| 作者单位： | 南京师范大学生命科学学院，南京师范大学生命科学学院，南京师范大学生命科学学院，南京师范大学生命科学学院 南京，210097，南京，210097，南京，210097，南京，210097 |
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| 基金项目： | 南京师范大学青年科学基金资助项目 (2 0 0 1XQ2 3) |
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| 摘 要： | 采用聚丙烯酰胺凝胶电泳技术，对刺猬（Erinaceus dealbatus）及游蛇科的黑眉锦蛇（Elaphe taeniura）、红点锦蛇（E.rufodorsata）和赤链蛇（Dinodon rufozonatum）等在冬眠期和活动期骨骼肌、心肌、肝等的乳酸脱氢酶（lactate dehydrogenase,LDH）同工酶谱进行了分析，并用比色法测定了以上各种组织的LDH活性。结果表明：LDH同工酶的电泳图谱只有红点锦蛇的肝和骨骼肌中存在活动期和冬眠期的明显差异，在其余动物两个时期的组织中未表现出明显变化；刺猬的肝脏LDH活性在冬眠期显著地高于活动期，而其余的动物组织冬眠期LDH活性均显著低于活动期；三种蛇类组织LDH4均未见表达。
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| 关 键 词： | 刺猬 游蛇科 冬眠期 活动期 乳酸脱氢酶 同工酶 比色法 酶活性 糖代谢 |
A Comparative study on the Lactate Dehydrogenase Isozymes in Tissues of Erinaceus dealbatus and Three species in Colubridae during Hibernation and Activity Period |
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| Ren Wenhua,Yang Guang,Gu Yu,Zhao Xiangwei.A Comparative study on the Lactate Dehydrogenase Isozymes in Tissues of Erinaceus dealbatus and Three species in Colubridae during Hibernation and Activity Period[J].Journal of Nanjing Normal University(Natural Science Edition),2002,25(1):20-23. |
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| Authors: | Ren Wenhua Yang Guang Gu Yu Zhao Xiangwei |
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| Abstract: | The lactate dehydrogenase (LDH) isozyme for three kinds of tissue (heart,liver,and skeletal muscle) from Erinaceus dealbatus,Elaphe taeniura,E.rufodorsata,Dinodon rufozonatum ,and Ptyas korros was analyzed by polyacrylamide gel electrophoresis.The total LDH enzemy activity were determined by colorimetry.The results dicate that there was nearly no difference from hibernation and activity period in all tissures except the liver and skeletal muscle of Elaphe rufodorsata as shown by electrophoresis plot,hibernation was lower than in activity period except that in liver of Erinaceus dealbatus ,and LDH 4 was not detected in all detected tissues of the three species of snakes. |
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| Keywords: | Erinaceus dealbatus Colubridae hibernation lactate dehydrogenase (LDH) |
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