| 一种新型叶绿体表达系统的构建 |
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| 引用本文： | 杨 捷,Michele M Champagne,A David Hieber,Adelheid R Kuehnle.一种新型叶绿体表达系统的构建[J].福州大学学报(自然科学版),2010,38(4):601-605. |
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| 作者姓名： | 杨 捷 Michele M Champagne A David Hieber Adelheid R Kuehnle |
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| 作者单位： | 福州大学生物科学与工程学院， 福建 福州 350108;Kuehnle AgroSystems, Inc, Honolulu, HI 96822, USA;Cardax Pharmaceuticals, Inc, Aiea, HI 96701, USA;Kuehnle AgroSystems, Inc, Honolulu, HI 96822, USA |
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| 摘 要： | 针对目前的叶绿体转基因技术存在载体构建步骤繁琐耗时的问题,建立了一种新型的不需自带启动子的叶绿体表达系统,选择模式植物烟草叶绿体中的假基因作为外源基因的插入位点.通过基因枪转化法获得4株独立的叶绿体转化植株.此新技术可实现叶绿体载体构建的时间、技术成本最小化,从而促进转基因叶绿体的应用.
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| 关 键 词： | 叶绿体 转化 烟草 假基因 |
Construction of a novel chloroplast expression system |
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| YANG Jie,Michele M Champagne,A David Hieber and Adelheid R Kuehnle.Construction of a novel chloroplast expression system[J].Journal of Fuzhou University(Natural Science Edition),2010,38(4):601-605. |
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| Authors: | YANG Jie Michele M Champagne A David Hieber and Adelheid R Kuehnle |
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| Institution: | College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China;Kuehnle AgroSystems, Inc, Honolulu, HI 96822, USA;Cardax Pharmaceuticals, Inc, Aiea, HI 96701, USA;Kuehnle AgroSystems, Inc, Honolulu, HI 96822, USA |
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| Abstract: | The current chloroplast transformation technology requires exogenous regulatory elements (promoters and terminators) to control the expression of foreign genes, and cloning of exogenous regulatory elements is laborious and time-consuming. This research established a novel chloroplast expression system in the model plant tobacco, utilizing a pseudogene as the targeted insertion site for foreign genes without exogenous promoters or terminators. Four independent transplastomic lines were obtained via biolistic bombardment. This new chloroplast transformation technology can minimize the time and cost of chloroplast vector construction and therefore promises to promote the applications of transgenic chloroplasts. |
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| Keywords: | chloroplast transformation tobacco pseudogene |
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