| Probing the Proteolysis of Melitin Using Liquid Secondary Ion Mass Spectrometry |
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| 引用本文： | 武轶,王敬尊,隋森芳. Probing the Proteolysis of Melitin Using Liquid Secondary Ion Mass Spectrometry[J]. 清华大学学报, 1997, 0(1) |
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| 作者姓名： | 武轶 王敬尊 隋森芳 |
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| 作者单位： | Wu Yi +，Wang Jingzun，Sui Senfang + +Department of Biological Sciences and Biotechnology，State Key Laboratory of Biomembrane and Membrane Biotechnology，Tsinghua University，Beijing 100084 Beijing Institute of Microchemis |
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| 摘 要： | ＰｒｏｂｉｎｇｔｈｅＰｒｏｔｅｏｌｙｓｉｓｏｆＭｅｌｉｔｉｎＵｓｉｎｇＬｉｑｕｉｄＳｅｃｏｎｄａｒｙＩｏｎＭａｓｓＳｐｅｃｔｒｏｍｅｔｒｙ＊ＷｕＹｉ＋（武轶），ＷａｎｇＪｉｎｇｚｕｎ（王敬尊），ＳｕｉＳｅｎｆａｎｇ＋（隋森芳）＊＋Ｄｅｐａｒｔｍｅ...
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Probing the Proteolysis of Melittin Using Liquid Secondary Ion Mass Spectrometry * |
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| Abstract: | Liquid secondary ion mass spectrometry(LSIMS) was used to probe the proteolysis of melittin by trypsin and pepsin. The results showed that LSIMS is good for monitoring proteolytic reactions. It can not only identify the proteolytic products of proteins, but also be used to research the dynamics of proteolytic reactions. The proteolysis of melittin by trypsin gave seven main peptide fragments: 1, AA 1 AA 7 ; 2, AA 1 AA 21 ;3, AA 1 AA 22 ; 4, AA 1 AA 23 ; 5, AA 1 AA 24 ; 6, AA 8 AA 21 ; 7, AA 8 AA 22 . The proteolysis of melittin with pepsin gave thirteen peptide fragments: 1, AA 1 AA 3; 2, AA 4 AA 6;3, AA 1 AA 4;4, AA 1 AA 5 ;5, AA 1 AA 6 ;6, AA 1 AA 8;7, AA 7 AA 13 ;8, AA 10 AA 13 ;9, AA 14 AA 16 ;10, AA 14 AA 26 ;11, AA 15 AA 26 ;12, AA 17 AA 26 ;13, AA 20 AA 26 . |
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| Keywords: | melittin proteolysis liquid secondary ion mass spectrometry |
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