| 罗汉果性别相关的RAPD标记 |
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| 引用本文： | 秦新民,黄夕洋,蒋水元.罗汉果性别相关的RAPD标记[J].广西师范大学学报(自然科学版),2007,25(3):109-112. |
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| 作者姓名： | 秦新民 黄夕洋 蒋水元 |
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| 作者单位： | 1. 广西师范大学,生命科学学院,广西,桂林,541004
2. 中国科学院,广西植物研究所,广西,桂林,541006 |
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| 基金项目： | 广西自然科学基金资助项目(桂科自0542040) |
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| 摘 要： | 应用随机扩增多态性DNA(RAPD)技术寻找与罗汉果性别相关的分子标记,筛选了130个10 bp的随机引物,发现有4个引物(S60、S90、S100、S343)能在雌、雄株DNA间扩增出5条差异性片段,大小在300~1 300 bp之间,表明这些特异带可被用来作为性别鉴别的特异性分子遗传标记。
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| 关 键 词： | 罗汉果 性别 RAPD标记 |
| 文章编号： | 1001-6600（2007）03-0109-04 |
| 收稿时间： | 2007-03-08 |
| 修稿时间： | 2007年3月8日 |
RAPD Markers Linked to Sex in Siraitia grosvenorii(Swingle)C. Jeffrey |
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| QIN Xin-min,HUANG Xi-yang,JIANG Shui-yuan.RAPD Markers Linked to Sex in Siraitia grosvenorii(Swingle)C. Jeffrey[J].Journal of Guangxi Normal University(Natural Science Edition),2007,25(3):109-112. |
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| Authors: | QIN Xin-min HUANG Xi-yang JIANG Shui-yuan |
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| Institution: | 1. College of Life Science, Guangxi Normal University, Guilin 541004,Chinas2. Guangxi Institute of Botany, Chinese Academy of Sciences,Guilin 541006, China |
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| Abstract: | The sex-linked molecule markers in Siraitia grosvenorii(Swingle)C.Jeffrey were studied by Random Amplified Polymorphic DNA(RAPD) analysis.The results showed that 4 random primers could amplify polymorphic markers of sex from genomic DNA of male and female plants.The primers S60,S90,S100,and S343,produced 5 male-specific PCR products ranged in size from 300 bp to 1 300 bp in RAPD aralysis.These markers could be used to differentiale male and female individuales of S.grosvenorii plants. |
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| Keywords: | Siraitia grosvenorii(Swingle)C Jeffrey sex RAPD marker |
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