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Use of3H-QNB in the isolation of plasma membrane from smooth muscle of the urinary bladder: Effect of oxalate on calcium uptake by the membrane fractions
Authors:S Batra
Institution:(1) Department of Obstetrics and Gynecology, University of Lund and AB Leo Research Laboratories, Box 941, S-25109 Helsingborg, (Sweden)
Abstract:Summary Specific binding of tritiated quinuclidinyl benzilate (3H-QNB) to surface membrane muscarinic receptors was utilized to identify plasma membrane (PM) fractions from smooth muscle of the rabbit urinary bladder. Accumulation of3H-QNB in the PM fraction was 4–5-fold higher than that in fractions of endoplasmic reticulum (EM) or mitochondria (M). A similar pattern of
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