| 植物生长调节剂对黄芪愈伤组织中次生代谢物质含量的影响 |
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| 引用本文： | 刘同祥,步营,张宗申.植物生长调节剂对黄芪愈伤组织中次生代谢物质含量的影响[J].中央民族大学学报(自然科学版),2008,17(3):77-82. |
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| 作者姓名： | 刘同祥 步营 张宗申 |
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| 作者单位： | [1]中央民族大学中国少数民族传统医学研究中心,北京100081; [2]大连工业大学生物与食品工程学院,大连116034 |
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| 基金项目： | 辽宁省教育厅资助项目，辽宁省大连市青年科技人才基金 |
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| 摘 要： | 以膜荚黄芪幼叶为外植体，运用正交设计研究2，4-D、6-BA和NAA等3种植物生长调节剂组合对愈伤组织生产黄芪黄酮和黄芪皂苷的影响．结果表明对黄芪黄酮合成的影响顺序是：NAA〉6-BA〉2，4-D；对黄芪皂苷合成的影响顺序是：2，4-D〉6-BA〉NAA．生产黄芪黄酮和黄芪皂苷的最佳培养基分别为；MS＋2，4-D（0．5mg·L^-1）＋6-BA（1．0mg·L^-1）＋NAA（2．0mg·L^-1和MS＋2，4-D（2．0mg·L^-1）＋6-BA（2．0mg·L^-1）＋NAA（1．0mg·L^-1）．细胞伸长有利于愈伤组织中次生代谢物质的合成．
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| 关 键 词： | 膜荚黄芪 植物生长调节剂 愈伤组织 正交设计 黄芪黄酮 黄芪皂苷 |
Effect of Plant Growth Regulators on the Content of Secondary Metabolites in Astragalus membranaceus(Fisch.)Bge.callus |
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| LIU Tong-xiang,BU Ying,ZHANG Zong-shen.Effect of Plant Growth Regulators on the Content of Secondary Metabolites in Astragalus membranaceus(Fisch.)Bge.callus[J].Journal of The Central University for Nationalities(Natural Sciences Edition),2008,17(3):77-82. |
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| Authors: | LIU Tong-xiang BU Ying ZHANG Zong-shen |
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| Institution: | LIU Tong-xiang , BU Ying,ZHANG Zong-shen ( 1. Chinese Minority Traditional Medical Center, Central University for Nationalities, Beijing 100081, China ; 2..School of Biology and Food Engineering, Dalian Polytechnic University, Dalian Liaoning 116034, China ) |
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| Abstract: | Taking the leaves of Astragalus membranaceus （ Fisch. ） Bge. as explants, the effects of 2,4- D,6-BA and NAA on production of flavonoids of astragalus and saponins of astragalus were studied by using orthogonal design. The yield of total flavonoids of astragalus and total saponins of astragalus were used as criteria of estimating the efficiency of three regulators, the results showed that the effects of regulators on total flavonoids of astragalus content was as ： NAA 〉 6-BA 〉 2,4-D, and the effect of regulators on total saponins of astragalus was as： 2,4-D 〉 6-BA 〉 NAA. The optimal culture medium for production of total flavonoids of astragalus was ： MS ＋ 2,4-D （0.5 mg·L^-1） ＋ 6-BA （ 1.0 mg·L^-1 ） ＋ NAA （2.0 mg·L^-1 ）, and that for production of total saponins of astragalus was as ： MS ＋ 2,4-D （2.0 mg·L^-1 ） ＋ 6-BA（2.0 mg·L^-1） ＋ NAA（ 1.0 mg·L^-1）. Cells elongation facilitated the synthesis of secondary metabolites in callus. |
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| Keywords: | Astragalus membranaceus （ Fisch ） Bge plant growth regulators callus orthogonal design total flavonoids of astragalus total saponins of astragalus |
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