| 活化琼脂糖多胺化材料作为基因的转染载体 |
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| 引用本文： | 张灵敏,储彬,彭晓慧,胡惠勇,汤顺清.活化琼脂糖多胺化材料作为基因的转染载体[J].暨南大学学报,2010,31(1). |
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| 作者姓名： | 张灵敏 储彬 彭晓慧 胡惠勇 汤顺清 |
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| 作者单位： | 曁南大学生物医学工程研究所,广东,广州,510632 |
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| 基金项目： | 国家863项目(2007AA09Z436) |
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| 摘 要： | 将琼脂糖氧化降解,再用环氧氯丙烷活化琼脂糖,与乙二胺反应引入胺基,最后结合pEGFP-C1.红外光谱、元素分析法、琼脂糖凝胶电泳和zeta电位分析实验表征了胺化琼脂糖的性质及其作为基因载体的效果.红外光谱及元素分析表明琼脂糖成功连接胺基;琼脂糖凝胶电泳显示胺化琼脂糖能够有效保护基因.以上结果为胺化琼脂糖制备基因载体提供了依据.
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| 关 键 词： | 基因载体 质粒 琼脂糖 |
Amination of epoxy-containing agarose as a gene transfection vector |
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| ZHANG Ling-min,CHU Bin,PENG Xiao-hui,HU Hui-yong,TANG Shun-qing.Amination of epoxy-containing agarose as a gene transfection vector[J].Journal of Jinan University(Natural Science & Medicine Edition),2010,31(1). |
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| Authors: | ZHANG Ling-min CHU Bin PENG Xiao-hui HU Hui-yong TANG Shun-qing |
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| Institution: | Biomedical Engineering Institute;Jinan University;Guangzhou 510632;China |
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| Abstract: | Agarose was degraded by oxidation,activated by chloroepoxypropane,and then aminated with ethylenediamine to introduce amino groups into agarose chain.Finally,the aminated agarose was combined to pEGFP-C1 plasmid.The infrared spectrophotometry analysis(IR),element analysis(EA),agarose gel electrophoresis,particle size and zeta potential analysis were carried out to characterize the as-prepared aminated agaorse and its application as a gene vector.The results of IR and EA showed that the agarose succeeded to ... |
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| Keywords: | gene vector plasmid agarose |
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