|
|||||
|
|
| 单壁碳纳米管对长片段聚合酶链式反应(PCR)增效机理的初步研究 | |
| 引用本文： | 刘清岱,王志伟,李光壁,王玉荣,安红杰.单壁碳纳米管对长片段聚合酶链式反应(PCR)增效机理的初步研究[J].天津科技大学学报,2012(2):1-4. |
| 作者姓名： | 刘清岱 王志伟 李光壁 王玉荣 安红杰 |
| 作者单位： | 1. 天津科技大学食品工程与生物技术学院,天津 300457 2. 天津科技大学海洋科学与工程学院,天津 300457 3. 阿德雷德大学环境科学学院,澳大利亚 SA5005 |
| 基金项目： | 国家自然科学基金资助项目(30900339,30800255,31101357);中国博士后科学基金(20110490786,2011M500528) |
| 摘 要： | 为了开发新型基因扩增技术,采用两步PCR(two-step PCR)和rpsL保真度实验等方法,对单壁碳纳米管(single-wall carbon nanotube,SWCNT)在长片段PCR反应中的增效机理进行研究.结果表明:在长片段PCR反应中,单壁碳纳米管最适工作质量浓度为0.8,mg/mL;单壁碳纳米管可以在较短的延伸时间增加扩增产物产量,提高DNA聚合酶的反应效率;通过rpsL保真度实验显示,对照组PCR产物的错误率为5.32×10–6,而实验组的错误率仅为2.39×10–6. |
| 关 键 词： | PCR 单壁碳纳米管 保真度 |
A Preliminary Study of Synergistic Mechanisms of Single-Wall Carbon Nanotube in Long-PCR |
|
| LIU Qingdai,WANG Zhiwei,LI Guangbi,WANG Yurong,AN Hongjie.A Preliminary Study of Synergistic Mechanisms of Single-Wall Carbon Nanotube in Long-PCR[J].Journal of Tianjin University of Science & Technology,2012(2):1-4. | |
| Authors: | LIU Qingdai WANG Zhiwei LI Guangbi WANG Yurong AN Hongjie |
| Institution: | 1,3(1.College of Food Engineering and Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China;2.College of Marine Science and Engineering,Tianjin University of Science & Technology,Tianjin 300457,China;3.School of Environmental Science,University of Adelaide,Australia SA 5005) |
| Abstract: | The effect of single-wall carbon nanotube(SWCNT)on long-PCR was studied with a two-step PCR and rpsL fidelity assay.The experiments results showed that the best concentration of SWCNT was 0.8,mg/mL in long-PCR system.SWCNT did contribute to the high activity of DNA polymerase,so an amplified DNA product was observed even within a very short reaction time.The error frequency of PCR products was 5.32×10-6in the control group,while the error frequency declined to 2.39×10-6by using SWCNT. |
| Keywords: | PCR single-wall carbon nanotube fidelity |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |