| 中药中速灭威残留量的酶联免疫检测方法 |
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| 引用本文： | 张曙光,王俊平,生威,张燕,王硕.中药中速灭威残留量的酶联免疫检测方法[J].天津科技大学学报,2012,27(3):21-24. |
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| 作者姓名： | 张曙光 王俊平 生威 张燕 王硕 |
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| 作者单位： | 食品营养与安全教育部重点实验室,天津科技大学食品工程与生物技术学院,天津300457 |
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| 摘 要： | 建立了直接竞争酶联免疫法(ELISA)测定金银花、枸杞、人参、板蓝根、大青叶和圆弧止痛片为代表的中药中速灭威的残留量.金银花等经过粉碎,加入甲醇提取、旋蒸、PBS复溶等简单前处理之后,再经过适度的稀释可以达到消除基质影响,用ELISA进行测定.中药样品添加回收率为64.00%～91.30%,变异系数均小于30.12%.结果表明,该方法可以简单、快速、灵敏、准确地检测出金银花、枸杞、人参、板蓝根、大青叶和圆弧止痛片等中药中速灭威的残留量.
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| 关 键 词： | ELISA 中药 速灭威 检测 |
Metolcarb Residue Analysis Using ELISA in Chinese Herbal Medicines |
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| ZHANG Shuguang
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WANG Junping
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SHENG Wei
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ZHANG Yan
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WANG Shuo.Metolcarb Residue Analysis Using ELISA in Chinese Herbal Medicines[J].Journal of Tianjin University of Science & Technology,2012,27(3):21-24. |
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| Authors: | ZHANG Shuguang WANG Junping SHENG Wei ZHANG Yan WANG Shuo |
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| Institution: | (Key Laboratory of Food Nutrition and Safety,Ministry of Education,College of Food Engineering and Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China) |
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| Abstract: | A direct competitive ELISA was established in detecting Metolcarb residues in Chinese herbal medicines,such as Lonicera chrysantha,Lycium chinense,Radix Ginseng,Radix Isatidis,Folium Isatidis and Yuanhu painkillers.After Lonicera chrysantha and so on were mashed and homogenized,methanolextraction,rotary steaming,and PBS were used to make up to volume.Moderate dilution then could eliminate the influence of matrix,which was detected by ELISA.In Chinese herbal medicines,the recovery rate was 66%-91%,with variation coefficient less than 30%.The method is simple,quick,sensitive and accurate in detecting the Metolcarb residues in Chinese herbal medicines. |
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| Keywords: | ELISA Chinese herbal medicines Metolcarb determination |
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