利用斑马鱼模型研究芦荟大黄素对宫颈癌细胞SiHa的抑制作用

通过细胞增殖实验（MTT）研究不同浓度芦荟大黄素（Aloe emodin，AE）对人宫颈癌细胞SiHa生长的抑制作用，并将SiHa细胞注入48 hpf的斑马鱼胚胎卵黄囊，建立斑马鱼移植瘤模型，然后用不同浓度AE进行处理，考察肿瘤细胞在斑马鱼体内的增殖和迁移情况。结果显示，AE对SiHa细胞的生长抑制率随浓度和作用时间的增加而呈明显上升趋势，表现出一定的浓度 时间 效应依赖关系；体内实验表明AE对斑马鱼移植瘤的增殖和转移具有抑制作用。本研究表明，AE具有开发成抗宫颈癌药物的潜能，同时斑马鱼移植瘤模型也可以成为宫颈癌研究和药物评价的重要模型。

Inhibitory effect of aloe emodine on cervical cancer cell line SiHa with zebrafish xenograft model

We employ MTT to investigate inhibitory effect of different concentrations of aloe emodin (AE) on the proliferation of cervical cancer cell SiHa. We further establish zebrafish xenograft model by injecting SiHa into the yolk sac of 48 hpf zebrafish. The zebrafish is then treated with different concentrations of AE to investigate proliferation and migration of cancer cell in zebrafish. Results show that growth inhibition rate of AE on SiHa significantly increases with the increase of AE concentration and action time, a certain concentration time effect dependence. In vivo experiment shows that AE has inhibitory effect on the proliferation and metastasis of zebrafish xenograft. Our research indicates that AE has the potential of cervical anticancer drug. The zebrafish xenograft model can also be an important model of cervical cancer research and its drug assessment.